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PE anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody

Pricing & Availability
Clone
4B2.9 (See other available formats)
Regulatory Status
RUO
Other Names
Vγ1 and Vγ2 (Tonegawa nomenclature), TRGV1, TRGV2, GV5S1, GV5S2
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
4B2.9_PE_1_112311
C57BL/6 mouse splenocytes were stained with CD3 APC and TCR Vγ1.1 + Vγ1.2 (clone 4B2.9) PE (top) or Armenian hamster IgG PE isotype control (bottom).
  • 4B2.9_PE_1_112311
    C57BL/6 mouse splenocytes were stained with CD3 APC and TCR Vγ1.1 + Vγ1.2 (clone 4B2.9) PE (top) or Armenian hamster IgG PE isotype control (bottom).
  • 4B2.9_PE_2_112311
See PE spectral data
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142703 25 µg 104€
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142704 100 µg 215€
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Description

In mice, the TCR γ locus consists of seven Vγ, four Jγ, and four Cγ genes, which are differentially expressed throughout their lifespan. During fetal and early postnatal life, very few γ/δ T cells express TCR Vγ1.1 or Vγ1.2 chains. In adult mice, however, a major fraction of the γ/δ T cell repertoire comprises either TCR Vγ1.1 or Vγ1.2 chains. The Vγ1.1 gene rearranges almost exclusively with the Jγ4-Cγ4 genes, while the Vγ1.2 gene preferentially rearranges with Jγ1.

The 4B2.9 antibody reacts specifically with mouse TCR Vγ1.1 and Vγ1.2 as designated by the Garman nomenclature system. Mouse TCR Vγ1.1 and Vγ1.2 are also known as TCR Vγ1 and Vγ2, respectively, under the Heilig & Tonegawa nomenclature system.

Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
TCR Vγ1.1/Vδ6 T3.13.1 hybridoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

mAb 4B2.9 specifically recognizes γ/δ T cells expressing Vγ1.1 and/or Vγ1.2 chains.

Application References

(PubMed link indicates BioLegend citation)
  1. Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)
Product Citations
  1. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  2. Bachy E, et al. 2016. J Exp Med. 213: 841 - 857. PubMed
RRID
AB_10960739 (BioLegend Cat. No. 142703)
AB_10960739 (BioLegend Cat. No. 142704)

Antigen Details

Distribution

Subset of γ/δ T cells

Function
Antigen recognition
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Garcia-Herrera A, et al. 2011. Am. J. Surg. Pathol. 35:1214.
2. Gutierrez A, et al. 2010. J. Clin. Oncol. 28:3816.
3. Zhang X, et al. 2009. Hematology 14:237.

Gene ID
110067 View all products for this Gene ID
UniProt
View information about TCR Vgamma1.1 1.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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