PE anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Product Details

Verified Reactivity

Rat, Mouse

Reported Reactivity

Rabbit, Guinea Pig

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte Thy-1 antigen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.

Application References

(PubMed link indicates BioLegend citation)

1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
4. Eitner F. 1997. Kidney. Int. 51:69.
5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
9. Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)

Product Citations

1. Yan H, et al. 2020. Adv Sci (Weinh). 7:1903516. PubMed
2. Chen M, et al. 2021. Bioact Mater. 1.591666667. PubMed
3. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
4. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
5. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
6. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
7. Yang J, et al. 2022. Dis Markers. 2022:7071877. PubMed
8. Sacirbegovic F 2023. Immunity. N/A. PubMed
9. Abu Hejleh AP, et al. 2023. Int J Tryptophan Res. 16:11786469231153111. PubMed
10. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
11. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
12. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
13. McGee MC, et al. 2022. Bio Protoc. 12:. PubMed
14. Lu JH, et al. 2023. J Funct Biomater. 14:. PubMed
15. Vukovic N, et al. 2023. Cancer Res Commun. 3:109. PubMed
16. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
17. Wang D, et al. 2018. Immunity. 48:659. PubMed
18. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
19. Nurbaeva MK, et al. 2018. Front Physiol. 0.93125. PubMed
20. Nicolas P, et al. 2022. J Exp Med. 219:. PubMed
21. Lai G, et al. 2022. PeerJ. 10:e13744. PubMed
22. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
23. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
24. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
25. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
26. Olson M, et al. 2012. PLoS Pathog. 8:e1003054. PubMed
27. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
28. Paschalis E, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.005. PubMed
29. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
30. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
31. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
32. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
33. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
34. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
35. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
36. Alsughayyir J, et al. 2019. Front Immunol. 2.485416667. PubMed
37. Zhang XW, et al. 2021. Oncoimmunology. 10:1920739. PubMed
38. Pauken K, et al. 2013. Diabetes. 62:2859. PubMed
39. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
40. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
41. Cizkova D, et al. 2014. Sci Rep. 4:7514. PubMed
42. O’Connor R, et al. 2010. J Immunol. 185:7235. PubMed
43. Hsu M, et al. 2019. Nat Commun. 10:229. PubMed

RRID

AB_1595524 (BioLegend Cat. No. 202523)
AB_1595524 (BioLegend Cat. No. 202524)

Antigen Details

Structure

Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD

Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

Function

Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells

Ligand/Receptor

Interacts with CD45

Cell Type

B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID

21838 View all products for this Gene ID 24832 View all products for this Gene ID

UniProt

View information about CD90/CD90.1 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis