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PE/Cyanine5 anti-human CD272 (BTLA) Antibody

Pricing & Availability
Clone
MIH26 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MIH26_PECyanine5_CD272_Antibody_1_120623
Human peripheral blood lymphocytes were stained with CD3 Alexa Fluor® 488 and CD272 (BTLA) (clone MIH26) PE/Cyanine5 (left), or mouse IgG2a, κ PE/Cyanine5 isotype control (right).
  • MIH26_PECyanine5_CD272_Antibody_1_120623
    Human peripheral blood lymphocytes were stained with CD3 Alexa Fluor® 488 and CD272 (BTLA) (clone MIH26) PE/Cyanine5 (left), or mouse IgG2a, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
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344531 25 tests 132€
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344532 100 tests 320€
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Description

B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It has been reported that BTLA may regulate T cell function through binding to B7-H4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BTLA transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell proliferation and cytokine production1. Clone MIH26 has agonistic activity on BTLA, resulting in the inhibition of activation.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for use in in vitro and in vivo biofunctional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121.
  2. Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.
RRID
AB_2819976 (BioLegend Cat. No. 344531)
AB_2819976 (BioLegend Cat. No. 344532)

Antigen Details

Structure
Ig superfamily, with similar structure to CTLA-4 and PD-1
Distribution

B cells and T lymphocytes, NK cells and dendritic cells

Function
Negative regulation of T cell activation, proliferation and cytokine production
Ligand/Receptor
HVEM
Cell Type
B cells, Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
2. Sun Y, et al. 2009. J. Immunol. 183:1946.
3. Gonzalez LC, et al. 2005. P. Natl. Acad. Sci. USA 102:1116.

Gene ID
151888 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org
Go To Top Version: 1    Revision Date: 12.23.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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