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PE/Cyanine7 anti-mouse Tim-4 Antibody

Pricing & Availability
Clone
RMT4-54 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein-4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMT4-54_PECyanine7_TIM-4_Antibody_101524
Balb/c peritoneal macrophages were stained with CD11b APC and TIM-4 (clone RMT4-54) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • RMT4-54_PECyanine7_TIM-4_Antibody_101524
    Balb/c peritoneal macrophages were stained with CD11b APC and TIM-4 (clone RMT4-54) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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130009 25 µg 118€
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130010 100 µg 306€
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Description

Tim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 binds to Tim-1 to promote T cell proliferation by enhancing cell division and reducing apoptosis. Tim-4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIM4-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2009. Blood. 113:3821. (FA)
  2. Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
Product Citations
  1. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  2. Zaman R, et al. 2021. Immunity. :. PubMed
  3. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  4. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  5. Wang Y, et al. 2022. Cell Rep. 41:111738. PubMed
  6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  7. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  8. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  9. De Simone G, et al. 2021. Immunity. :. PubMed
  10. Blriot C, et al. 2021. Immunity. :. PubMed
  11. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  12. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  13. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  14. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
RRID
AB_2565718 (BioLegend Cat. No. 130009)
AB_2565718 (BioLegend Cat. No. 130010)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution

Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.

Function
Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Interaction
Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
Ligand/Receptor
Phosphatidylserine, TIM1
Cell Type
Antigen-presenting cells
Biology Area
Immunology
Antigen References

1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706

Gene ID
276891 View all products for this Gene ID
UniProt
View information about Tim-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.31.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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