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PE/Fire™ 744 anti-human CD80 Antibody

Pricing & Availability
Clone
W17149D (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, BB1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W17149D_PE-FIRE-744_CD80_FC_081224
Human peripheral blood mononuclear cells were stained with anti-human CD19 (clone HIB19) Alexa Fluor® 700 and anti-human CD80 (clone W17149D) PE/Fire™ 744 (left) or rat IgG2a, κ PE/Fire™ 744 isotype control (right).
  • a. W17149D_PE-FIRE-744_CD80_FC_081224
    Human peripheral blood mononuclear cells were stained with anti-human CD19 (clone HIB19) Alexa Fluor® 700 and anti-human CD80 (clone W17149D) PE/Fire™ 744 (left) or rat IgG2a, κ PE/Fire™ 744 isotype control (right).
  • b. W17149D_PE-FIRE-744_CD80_FC-2_081224
    Raji cell line was stained with anti-human CD80 (clone W17149D) PE/Fire™ 744 (filled histogram) or rat IgG2a, κ PE/Fire™ 744 isotype control (open histogram).
Compare all formats See PE/Fire™ 744 spectral data
Cat # Size Price Quantity Check Availability Save
375413 25 tests 137€
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375414 100 tests 300€
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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RBL1 cells stably transfected with human CD80 (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Lim TS, et al. 2012. PLoS One. 7(9): e45185.
2. Kamphorst, Alice O, et al. 2017. Science. 31;355(6332):1423-1427.
3. Zheng Y, et al. 2004. J. Immunol. 1;172(5):2778-84.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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