- Clone
- MH5A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD-1 homolog, Dies1, B7-H5
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MH5A_PEDZL594_PD-1H_Antibody_013018 MH5A_PEDZL594_PD-1H_Antibody_013018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MH5A_PEDZL594_PD-1H_Antibody_013018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD8a APC and PD-1H (clone MH5A) PE/Dazzle™ 594 (left) or Armenian Hamster IgG PE/Dazzle™ 594 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
143717 | 25 µg | 132€ | ||||
143718 | 100 µg | 306€ |
PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- PD-1H- IgG Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Flies DB, et al. 2011. J. Immunol. 187:1537.
- RRID
-
AB_2728162 (BioLegend Cat. No. 143717)
AB_2728163 (BioLegend Cat. No. 143718)
Antigen Details
- Structure
- Type I transmembrane protein, member of the CD28 family
- Distribution
-
Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
- Function
- Negative regulator of the immune response
- Cell Type
- Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Antigen References
-
1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577. - Gene ID
- 74048 View all products for this Gene ID
- UniProt
- View information about PD-1H on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All PD-1H Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse PD-1H (VISTA) | MH5A | FC,IHC-F,WB |
PE anti-mouse PD-1H (VISTA) | MH5A | FC |
FITC anti-mouse PD-1H (VISTA) | MH5A | FC |
APC anti-mouse PD-1H (VISTA) | MH5A | FC |
PerCP/Cyanine5.5 anti-mouse PD-1H (VISTA) | MH5A | FC |
PE/Cyanine7 anti-mouse PD-1H (VISTA) | MH5A | FC |
Brilliant Violet 421™ anti-mouse PD-1H (VISTA) | MH5A | FC |
Alexa Fluor® 647 anti-mouse PD-1H (VISTA) | MH5A | FC |
Alexa Fluor® 488 anti-mouse PD-1H (VISTA) | MH5A | FC |
PE/Dazzle™ 594 anti-mouse PD-1H (VISTA) | MH5A | FC |
Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA) | MH5A | FC,IHC-F,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 APC and puri... Balb/C mouse frozen spleen tissue slices were fixed with 4% ... -
PE anti-mouse PD-1H (VISTA)
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FITC anti-mouse PD-1H (VISTA)
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APC anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-... -
PerCP/Cyanine5.5 anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-... -
PE/Cyanine7 anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-... -
Brilliant Violet 421™ anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8a APC and PD-... -
Alexa Fluor® 647 anti-mouse PD-1H (VISTA)
57BL/6 mouse splenocytes were stained with CD8a PE and PD-1H... -
Alexa Fluor® 488 anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8a APC and PD-... -
PE/Dazzle™ 594 anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8a APC and PD... -
Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA)
C57BL/6 mouse splenocytes were stained with CD8 APC and puri...
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