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PerCP anti-human CD19 Antibody

Pricing & Availability
Clone
4G7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B4, CVID3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4G7_PerCP_CD19_Antibody_073118
Human peripheral blood lymphocytes were stained with CD19 (clone 4G7, filled histogram) PerCP or mouse IgG1, κ isotype control (open histogram) PerCP.
  • 4G7_PerCP_CD19_Antibody_073118
    Human peripheral blood lymphocytes were stained with CD19 (clone 4G7, filled histogram) PerCP or mouse IgG1, κ isotype control (open histogram) PerCP.
Compare all formats See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
392509 25 tests 128€
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392510 100 tests 278€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Cabezudo E, et al. 1999. Haematologica. 84:413-8
  2. Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
Product Citations
  1. Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed
  2. Xue D, et al. 2016. Front Immunol. 558:7. PubMed
  3. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
RRID
AB_2750100 (BioLegend Cat. No. 392509)
AB_2750100 (BioLegend Cat. No. 392510)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

Normal and malignant B lymphocytes

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Lymphocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Tedder T, et al. 1994. Immunol. Today 15:437.
  2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 07.31.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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