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PerCP/Cyanine5.5 anti-human CD16 Antibody

Pricing & Availability
Clone
B73.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B731_PerCPCyanine55_CD16_Antibody_012919
Human peripheral blood lymphocytes were stained with CD16 (clone B73.1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • B731_PerCPCyanine55_CD16_Antibody_012919
    Human peripheral blood lymphocytes were stained with CD16 (clone B73.1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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360711 25 tests 137€
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360712 100 tests 306€
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell-enriched fraction from human peripheral blood.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84.

Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Perussia B, et al. 1983. J. Immunol. 130:2133.
  2. Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
  3. Perussia B, et al. 1984. J. Immunol. 133:180.
  4. Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)
Product Citations
  1. Mittler E, et al. 2022. Sci Transl Med. 14:eabl5399. PubMed
  2. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  3. Chernyshev M, et al. 2023. Nat Commun. 14:2249. PubMed
  4. Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed
  5. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  6. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  7. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
RRID
AB_2562955 (BioLegend Cat. No. 360711)
AB_2562955 (BioLegend Cat. No. 360712)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
IgG Fc receptor III (FcγRIII)
Cell Type
Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Björkström NK, et al. 2008. J. Immunol. 181:4219.

Gene ID
2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01.29.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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