PerCP/Cyanine5.5 anti-human IgE Antibody anti-IgE

Pricing & Availability

Clone: MHE-18 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHE-18_PerCPCyanine55_IgE_Antibody_1_112024 — Human peripheral blood lymphocytes were stained with anti-human CD193 (clone 5E8) FITC and anti-human IgE (clone MHE-18) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).

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325511

25 tests

141€

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325512

100 tests

306€

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Description

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
RRID: AB_11219590 (BioLegend Cat. No. 325511)
AB_11219590 (BioLegend Cat. No. 325512)

Antigen Details

Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497 View all products for this Gene ID
UniProt: View information about IgE on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All IgE Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human IgE	MHE-18	FC,ELISA,IHC
Biotin anti-human IgE	MHE-18	FC,ELISA,IHC
PE anti-human IgE	MHE-18	FC
APC anti-human IgE	MHE-18	FC
PE/Cyanine7 anti-human IgE	MHE-18	FC
PerCP/Cyanine5.5 anti-human IgE	MHE-18	FC
Alexa Fluor® 647 anti-human IgE	MHE-18	FC
APC/Fire™ 750 anti-human IgE	MHE-18	FC
TotalSeq™-A0868 anti-human IgE	MHE-18	PG
APC/Cyanine7 anti-human IgE	MHE-18	FC
TotalSeq™-C0868 anti-human IgE	MHE-18	PG
TotalSeq™-B0868 anti-human IgE	MHE-18	PG

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Go To Top Version: 3 Revision Date: 05.24.2019

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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