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PerCP/Cyanine5.5 anti-mouse CD11a/CD18 (LFA-1) Antibody

Pricing & Availability
Clone
H155-78 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 1, ITGAL, ITGB2
Isotype
Rat IgG1, κ
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Product Citations
publications
H155-78_PerCPCyanine55_CD11a-CD18_Antibody_010319
C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cyanine5.5 (filled histogram) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • H155-78_PerCPCyanine55_CD11a-CD18_Antibody_010319
    C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cyanine5.5 (filled histogram) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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141007 25 µg 95€
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141008 100 µg 259€
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Description

Lymphocyte function-associated antigen 1 or LFA-1 is a heterodimer composed of two members of the integrin family, αL (CD11a) and β2 (CD18).  LFA-1 is expressed primarily on lymphocytes, monocytes/macrophages, and granulocytes.  It is involved in cell adhesion and costimulation processes. The ligands for LFA-1 are ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TH-derived anti-I-Ak CTL clone A1 5.1.17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.

Application References

(PubMed link indicates BioLegend citation)
  1. Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
Product Citations
  1. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  2. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  3. Dupont C, et al. 2015. J Immunol. 195: 4369 - 4377. PubMed
  4. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  5. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  6. Dupont C, et al. 2014. PLoS Pathog. 10:1004047. PubMed
  7. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  8. Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
  9. Czupalla CJ, et al. 2018. Bio Protoc. 8:. PubMed
RRID
AB_10694861 (BioLegend Cat. No. 141007)
AB_10694861 (BioLegend Cat. No. 141008)

Antigen Details

Structure
Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
Distribution

Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes

Function
Intercellular adhesion and costimulation
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
Cell Type
B cells, Dendritic cells, Lymphocytes, Macrophages, Monocytes
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID
16408 View all products for this Gene ID 16414 View all products for this Gene ID
UniProt
View information about CD11a CD18 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 01.03.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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