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PerCP/Cyanine5.5 anti-mouse/rat CD81 Antibody

Pricing & Availability
Clone
Eat-2 (See other available formats)
Regulatory Status
RUO
Other Names
TAPA-1
Isotype
Armenian Hamster IgG1, κ
Ave. Rating
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Product Citations
publications
Eat-2_PerCpCy5.5_CD81_Antibody_FC_120213.jpg
C57BL/6 mouse splenocytes were stained with mouse/rat CD81 (clone Eat-2) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • Eat-2_PerCpCy5.5_CD81_Antibody_FC_120213.jpg
    C57BL/6 mouse splenocytes were stained with mouse/rat CD81 (clone Eat-2) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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104911 25 µg 141€
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104912 100 µg 306€
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Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CD81+ mouse B lymphoma 38C13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2.

Application References

(PubMed link indicates BioLegend citation)

1. Maecker HT, et al. 2000. Hybridoma 19:15. (IP, WB, Stim)
2. Clark KL, et al. 2001. J. Immunol. 167:5115. (IP, WB, Activ)
3. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. PubMed
4. Castro-Seoane R, et al. 2012. PLoS Pathog. 8:1002538. PubMed

Product Citations
  1. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  2. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
RRID
AB_2562994 (BioLegend Cat. No. 104911)
AB_2562994 (BioLegend Cat. No. 104912)

Antigen Details

Structure
TM4SF tetraspanin, 26 kD
Distribution

T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts

Function
Regulates cell growth
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Levy S, et al. 1998. Annu. Rev. Immunol. 16:89.
3. Maeker HT, et al. 1997. FASEB J. 11:428.
4. Boismenu R, et al. 1996. Science. 271:198.

Gene ID
12520 View all products for this Gene ID 25621 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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