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- Clone
- A23002B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACACA, ACCα, ACC-Alpha, ACC1, Acetyl-Coenzyme A Carboxylase alpha, ACC
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Whole cell extracts (15 µg total protein) from HEK293T cells untreated (-) or treated with Oligomycin (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Acetyl-CoA Carboxylase Alpha Phospho (Ser79) (clone A23002B)(right panel) or Pan anti-Acetyl-CoA Carboxylase Alpha (left panel). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643808) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining with Purified anti-Acetyl-CoA Carboxylase Alpha Phospho (Ser79) (clone A23002B) was performed on formalin-fixed paraffin-embedded human liver tissue following antigen retrieval using Citrate Buffer (Cat. No. 420901). The tissue was incubated without (panel A) and with (panel B) Purified anti-Acetyl-CoA Carboxylase Alpha Phospho (Ser79) (clone A23002B), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 660051 | 25 µg | 128€ | ||||
| 660052 | 100 µg | 356€ | ||||
Acetyl-CoA carboxylase alpha (ACCα or ACACA) is a crucial enzyme in fatty acid biosynthesis. This enzyme catalyzes the conversion of acetyl-CoA to malonyl-CoA, a key step in the production of fatty acids. This enzyme plays a vital role in various cellular functions, including energy storage, membrane formation, and hormone production. The activity of Acetyl-CoA carboxylase alpha is tightly regulated by factors such as insulin, glucagon, and dietary fat intake, ensuring a balance between fatty acid synthesis and catalysis. Phosphorylation at serine 79 (Ser79) is a critical regulatory mechanism in response to changes in energy status and nutrient availability. This specific phosphorylation event inhibits the enzyme activity, thereby reducing the production of malonyl-CoA, a key precursor in fatty acid synthesis. Dysregulation of Acetyl-CoA carboxylase alpha is associated with several metabolic disorders, including obesity, type 2 diabetes, non-alcoholic fatty liver disease (NAFLD), and certain cancers. Hence, targeting Acetyl-CoA carboxylase alpha can help reduce lipid accumulation and alleviate mitochondrial dysfunction, making it a potential therapeutic target for these conditions.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic human Acetyl-CoA Carboxylase peptide phosphorylated at Serine 79
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use of this antibody on immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704) or Citrate Buffer (Cat. No. 420901).
Antigen Details
- Structure
- This enzyme is a 2346 amino acid protein with a molecular weight of approximately 265 kDa. It can exist as a monomer, homodimer, and homotetramer.
- Distribution
-
The Acetyl-CoA carboxylase alpha is located in the cytoplasm.
- Function
-
Function:
- Fatty Acid Biosynthesis: Catalyzes the conversion of acetyl-CoA to malonyl-CoA, a crucial step in fatty acid synthesis
- Energy Storage: Plays a role in storing energy by facilitating the production of fatty acids, which are later stored as triglycerides
- Membrane Formation: Contributes to the synthesis of fatty acids that are essential components of cell membranes
- Hormone Production: Involved in the biosynthesis of fatty acids that serve as precursors for various hormones
Regulation: -Phosphorylation:
- Inhibition: Phosphorylation at serine 79 (Ser79) inhibits ACCα activity, reducing fatty acid synthesis
- Activation: Dephosphorylation activates ACCα, promoting fatty acid synthesis
Hormonal Control:
- Insulin: Activates ACCα, enhancing fatty acid synthesis
- Glucagon and Epinephrine: Inhibit ACCα, reducing fatty acid synthesis
Nutrient Availability:
- High Carbohydrate Diet: Increases ACCα activity
- High Fat Diet: Decreases ACCα activity - Interaction
-
BRCA1: In its inactive phosphorylated form, ACCα interacts with the BRCT domains of BRCA1, which prevents ACCα dephosphorylation and inhibits lipid synthesis.
MID1IP1: This interaction promotes the oligomerization of ACCα, increasing its enzymatic activity.
AMPK (Adenosine Monophosphate-Activated Protein Kinase): Phosphorylates ACCα, leading to its inactivation and subsequent inhibition of lipid synthesis
Actin Filaments: ACCα can associate with actin filaments, which may help regulate its activity and localization. - Antigen References
-
- Dong J, et al. 2024. Journal of Translational Medicine. 22:196.
- Yang W, et al. 2024. BCM Med. 22:376.
- Xie J, et al. 2024. Front Biosci. 29:143.
- Jokinen MJ, et al. 2024. Trends Pharmacol Sci. 45:319.
- Gene ID
- 31 View all products for this Gene ID
- UniProt
- View information about Acetyl-CoA Carboxylase 1 on UniProt.org
Related FAQs
Other Formats
View All Acetyl-CoA Carboxylase 1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Acetyl-CoA Carboxylase alpha Phospho (Ser79) | A23002B | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Acetyl-CoA Carboxylase alpha Phospho (Ser79)
Whole cell extracts (15 µg total protein) from HEK293T cells... 
IHC staining with Purified anti-Acetyl-CoA Carboxylase Alpha...
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