IMPORTANT NOTICE: Our email addresses will soon change. Learn more >>
Loading...

Purified anti-Argonaute 3 Antibody

Pricing & Availability
Clone
W21012A (See other available formats)
Regulatory Status
RUO
Other Names
Argonaute-3, Argonaute RISC Catalytic Component 3, Eukaryotic Translation Initiation Factor 2C, 3, EIF2C3, HAGO3
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W21012A_PURE_-Argonaute-3_WB_010325
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Argonaute 3 (clone W21012A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W21012A_PURE_-Argonaute-3_WB_010325
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Argonaute 3 (clone W21012A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
651651 25 µg 120€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
651652 100 µg 308€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Argonaute 3 (AGO3) is part of the Argonaute protein family, which plays an important role in RNA silencing and gene regulation. In the RNA interference (RNAi) pathway, AGO3 interacts with miRNAs, helping regulate gene expression post-transcriptionally by binding to specific RNA targets and blocking their translation into proteins. Although AGO3, like AGO1 and AGO4, lacks significant catalytic activity, it still plays a role in RNA binding and miRNA stabilization, allowing it to assist in gene silencing. This process is crucial in a variety of biological functions, including cell differentiation, immune responses, and development.
 
Mutations or disruptions in AGO3 can have deleterious effects on reproductive health. AGO3 deficiency in Drosophila melanogaster (fruit flies) leads to a buildup of transposons in the germline and, consequently, sterility. This highlights AGO3's importance in maintaining reproductive viability by safeguarding genetic material.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Argonaute 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It is not recommended using this product in immunocytochemistry (ICC) or immunohistochemistry with FFPE tissue (IHC-P).

Antigen Details

Structure
Argonaute 3 is an 860 amino acid length protein with a predicted molecular weight of 97.3 kD and contains PAZ, Piwi, and guide RNA binding domains.
Distribution

Cytoplasmic

Function
Required for RNA-mediated gene silencing (RNAi)
Interaction
Interacts with EIF4B, IMP8, PRMT5 and TNRC6B
Biology Area
Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References
  1. Kirino Y, et al. 2009. Nat Cell Biol. 11:652-8.
  2. Nagao A, et al, 2010. RNA. 16:2503-15.
  3. Zhang Z, et al. 2016. Nat Plants. 2:16049.
Gene ID
192669 View all products for this Gene ID
UniProt
View information about Argonaute 3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.03.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account