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Purified anti-Biotin Antibody

Pricing & Availability
Clone
1D4-C5 (See other available formats)
Regulatory Status
RUO
Other Names
Vitamin B7, Vitamin H
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1D4-C5_Pure_071310
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), followed by purified 1D4-C5 and anti-mouse IgG PE
  • 1D4-C5_Pure_071310
    C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), followed by purified 1D4-C5 and anti-mouse IgG PE
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409002 500 µg 235€
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Description

Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Biotinylated limulus hemocyanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
  2. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  2. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  3. Jiang S, et al. 2022. Immunity. 55:1118. PubMed
  4. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  5. Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed
  6. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  7. Green N, et al. 2012. J Biol Chem. 287:39789. PubMed
RRID
AB_10642032 (BioLegend Cat. No. 409002)

Antigen Details

Ligand/Receptor
Avidin, Streptavidin
Gene ID
NA
UniProt
View information about Biotin on UniProt.org
Go To Top Version: 2    Revision Date: 12.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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