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Purified anti-BLK Antibody

Pricing & Availability
Clone
W21005C (See other available formats)
Regulatory Status
RUO
Other Names
Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-BLK
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W21005C_PURE_BLK_WB_102524
Human peripheral blood lymphocytes were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), intracellularly stained with Purified anti-BLK (clone W21005C), or Purified anti-rat IgG2b, κ Isotype Control (clone RTK4530) (Cat. No. 400602), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) and co-stained with PE anti-human CD19 (clone HIB19) (Cat. No. 302254).
  • A. W21005C_PURE_BLK_WB_102524
    Human peripheral blood lymphocytes were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), intracellularly stained with Purified anti-BLK (clone W21005C), or Purified anti-rat IgG2b, κ Isotype Control (clone RTK4530) (Cat. No. 400602), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) and co-stained with PE anti-human CD19 (clone HIB19) (Cat. No. 302254).
  • B. W21005C_PURE_BLK_ICFC_102524
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-BLK (clone W21005C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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642351 25 µg 128€
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642352 100 µg 356€
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Description

BLK is a non-receptor tyrosine kinase primarily expressed in B lymphocytes and other cells of the B-cell lineage. BLK belongs to the Src family of kinases and plays a critical role in B cell receptor signaling, which is essential for the activation, differentiation, and proliferation of B cells. Phosphorylation of target proteins by BLK helps transmit signals that guide B cell development and activation. Dysregulation of BLK has been associated with various immune disorders, including autoimmune diseases and certain types of B cell malignancies. Outside of B cells, BLK is also expressed in a variety of immune and non-immune cells including plasmacytoid dendritic cells, pancreatic β cells, and bone marrow progenitor cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full-length human BLK
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product (clone W21005C) may cross-react with other members of the Src kinase family.

This product (clone W21005C) is not recommended for use in immunocytochemistry (ICC) or immunohistochemistry (IHC).

For use in intracellular flow cytometry (ICFC), permeabilization with True-PhosTM Perm Buffer (Cat. No. 425401) is recommended. Fixation/permeabilization with Cyto-FastTM Fix/Perm Buffer Set (Cat. No. 426803) is not recommended.  

This antibody does not cross react with mouse BLK in Western Blot (WB).

Antigen Details

Structure
BLK is a 505 amino acid protein with a predicted molecular weight of 57.7 kD.
Distribution

Cytoplasmic, plasma membrane

Function
Kinase
Interaction
Phosphorylates a variety of targets including BLNK, IRF3, SYK, and ITAM-containing proteins such as CD79a and CD79b
Cell Type
B cells, Leukemia
Biology Area
Cell Biology, Immuno-Oncology, Immunology
Antigen References
  1. Dymecki SM, et al. 1990. Science. 247:332-6. 
  2. Zhang H, et al. 2012. Nat Genet. 44:861-71. 
  3. Malek SN, et al. 1998. Proc Natl Acad Sci USA. 95:7351-6. 
  4. Akerblad P, et al. 1999. J Immunol. 163:5453-61.
Gene ID
640 View all products for this Gene ID
UniProt
View information about BLK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.25.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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