- Clone
- A19010A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B Cell Linker (BLNK), B-Cell Adapter Containing ASH2 Domain Protein, SLP65, BASH
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
608351 | 25 µg | 108€ | ||||
608352 | 100 µg | 320€ |
B cell Linker protein (BLNK) is a cytoplasmic adaptor protein that is involved in B cell antigen receptor (BCR) signaling, a mechanism that is essential for the development of B lymphocytes in the bone marrow and periphery. Following BCR activation, BLNK undergoes tyrosine phosphorylation by Syk which induces interaction with effector molecules such as Grb2, Vav, and PLCγ to facilitate downstream signaling pathways. Phosphorylation sites include Tyr72, Tyr84, Tyr96, Tyr178, Tyr189, and Tyr394. Phosphorylation at Tyr96 residue facilitates BTK binding and plays a critical role in B cell maturation. BLNK mutations can block pro- to pre-B cell transitioning and result in gammaglobulinemia and absent B cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human BLNK phosphorylated at Tyrosine 96
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product has high background in WB if used at concentrations above 0.2 μg/mL.
This product is not suitable for ICC if cells are fixed with methanol only. - RRID
-
AB_2936688 (BioLegend Cat. No. 608351)
AB_2936688 (BioLegend Cat. No. 608352)
Antigen Details
- Structure
- BLNK is a 456 amino-acid protein with a predicted molecular weight of 50 kD.
- Distribution
-
BLNK is expressed in cytoplasm of B cells.
- Function
- Activation of BLNK plays a critical role in B cell proliferation, differentiation, and apoptosis.
- Interaction
- BLNK interacts with Grb2, Vav, PLCγ2, and Nck1.
- Cell Type
- B cells
- Biology Area
- Cell Biology
- Molecular Family
- Adaptor Proteins, Phospho-Proteins
- Antigen References
-
- Fu C, et al. 1998. Immunity. 9:93-103.
- Kurosaki T & Tsukada S. 2000. Immunity. 12:1-5.
- Gauld SB, et al. 2002. Science. 296:1641-2.
- Minegishi Y, et al. 1999. Science. 286:1954-7.
- Gene ID
- 29760 View all products for this Gene ID
- UniProt
- View information about BLNK Phospho Tyr96 on UniProt.org
Related FAQs
Other Formats
View All BLNK Phospho (Tyr96) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-BLNK Phospho (Tyr96) | A19010A | WB,ICC,ICFC |
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