- Clone
- Poly22A03 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyclic AMP-Responsive Element-Binding Protein 1 (Serine 133), CAMP Responsive Element Binding Protein 1
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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611651 | 50 µL | 244€ | ||||
611652 | 200 µL | 560€ |
Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1/2 in response to the activation of MAPK signaling.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Synthetic peptide of human CREB phosphorylated at Ser133
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:2000-1:3000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The CREB1 peptide phosphorylated at Serine 133 used for immunization has 100% homology with ATF-1 sequence phosphorylated at serine 63. Therefore, this clone will detect both phosphorylated proteins if present in testing sample.
- RRID
-
AB_3083400 (BioLegend Cat. No. 611651)
AB_3083400 (BioLegend Cat. No. 611652)
Antigen Details
- Structure
- CREB1 contains 327 amino acids at a predicted molecular weight at 43 kD
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
- Interaction
- CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
- Andre S, et al. 2020. Cell Mol Life Sci. 77:4049-4067.
- Pei-Hsuan L, et al. 2020. Diagnostics. 10:295.
- Kensuke S, et al. 2011. J Neurochem. 116:1-9.
- Andy YW, et al. 2010. J Immunol. 185:6413-6419.
- Arnould T, et al. 2002. EMBO J. 21:53-63.
- Shaista N, et al. 2014. Biochem J. 458:469-79.
- Gene ID
- 1385 View all products for this Gene ID
- UniProt
- View information about CREB1 Phospho Ser133 on UniProt.org
Related FAQs
Other Formats
View All CREB1 Phospho Ser133 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CREB1 Phospho (Ser133) | Poly22A03 | WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-CREB1 Phospho (Ser133)
Whole cell extracts (15 µg protein) from serum-starved HeLa ...
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