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Purified anti-E. coli RNA Polymerase β Antibody

Pricing & Availability
Clone
NT63 (See other available formats)
Regulatory Status
RUO
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
NT63_PURE_RNA_PolymeraseBeta_WB_102717_updated
Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone NT63). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder.
  • NT63_PURE_RNA_PolymeraseBeta_WB_102717_updated
    Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone NT63). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder.
  • NT63_PURE_RNA_PolymeraseBeta_WB_2_061418_updated
    Total cell lysate from E Coli. Strain BL21 (15 µg protein) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL (lane 1), 0.05 µg/mL (lane 2), 0.025 µg/mL (lane 3), 0.0125 µg/mL (lane 4) and 0.00625 µg/mL (lane 5) of Purified anti-E. coli RNA Polymerase β Antibody (clone NT63) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for anti-E. coli RNA Polymerase β Antibody (upper blot). Ponceau S staining (lower blot) was used as loading control. Lane M: Molecular Weight Marker
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663006 100 µg 259€
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. RNA polymerase β is a subunit which, along with α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified protein.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.00625 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone NT63 recognizes epitope 922-1090.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 1992. Biochemistry 31:7003.
Product Citations
  1. Wood TE, et al. 2019. Cell Rep. 29:187. PubMed
RRID
AB_2565555 (BioLegend Cat. No. 663006)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerard Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org
Go To Top Version: 3    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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