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Purified anti-Filamin A Antibody

Pricing & Availability
Clone
W18172E (See other available formats)
Regulatory Status
RUO
Other Names
Filamin A, Endothelial Actin-Binding Protein, Actin Binding Protein 280, Non-Muscle Filamin, Filamin A Alpha, Alpha-Filamin, Filamin-1, Filamin-A, ABP-280, FLN-A, FLN1, FLN, Filamin A Alpha (Actin Binding Protein 280),
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W18172E_PURE_Filamin-A_Antibody_1_072720
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-Filamin A antibody (clone W18172E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18172E_PURE_Filamin-A_Antibody_1_072720
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-Filamin A antibody (clone W18172E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18172E_PURE_Filamin-A_Antibody_2_072720
    Whole cell extracts (15 µg protein) from HeLa cells transfected with scramble control siRNA or siRNA targeting Filamin A were resolved on a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1 µg/mL of purified anti-Filamin A antibody (clone W18172E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. siRNA targeting Filamin A for depletion was obtained from Dharmacon (ON-TARGETplus FLNA (2316) siRNA - SMARTpool, Cat. No. L-012579-02-0005)
  • W18172E_PURE_Filamin-A_Antibody_3_072720
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes at 4°C, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5 µg/mL of either purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) (panel A) or purified anti-Filamin A antibody (clone W18172E) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
937701 25 µg 81€
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937702 100 µg 203€
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Description

Filamin A is an actin binding protein which crosslinks actin filaments into an orthogonal network and helps anchor membrane glycoproteins to cytoskeleton. Filamin A is also important in modifying cell shape in cell migration by changing the cytoskeleton. Filamin A is involved in ciliogenesis, cell adhesion and in the development of blood vessels, and other organs. Diseases associated with Filamin A include Melnick-Needles syndrome, otopalatodigital syndrome, periventricular nodular heterotopias and frontometaphyseal dysplasia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Filamin A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human and mouse Filamin A. 

Clone works well in ICC when cells are permeabilized with either Triton X-100 or methanol.

Clone is not recommended for IP.

RRID
AB_2876755 (BioLegend Cat. No. 937701)
AB_2876755 (BioLegend Cat. No. 937702)

Antigen Details

Structure
Filamin A is a 2647 amino acid protein with a predicted molecular weight of 281 kD.
Distribution

Filamin A is primarily localized in the actin filaments, plasma membrane and cytosol.

Function
Actin binding protein
Interaction
Actin, FLNB
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Feng Y, et al. 2006. Proc Natl Acad Sci U S A. 103:19836-41.
  2. Patel-Hett S, et al. 2011. Blood. 118:1641-52.
  3. Sun Y, et al. 2010. Am J Hum Genet. 87:146-53.
  4. Foley C, et al. 2010. Mol Syndromol. 1:121-126.
Gene ID
2316 View all products for this Gene ID
UniProt
View information about Filamin A on UniProt.org
Go To Top Version: 1    Revision Date: 07.27.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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