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Purified anti-GABABR2 Recombinant Antibody

Pricing & Availability
Clone
QA19A84 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-aminobutyric acid type B receptor subunit 2, gb2, GABA-BR2, GABA-B-R2, GABA-B receptor 2, GABA-B receptor, R2 subunit, G protein-coupled receptor 51, G-protein coupled receptor 51, gamma-aminobutyric acid B receptor 2, GABABR2, GABBR2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A84_PURE_GABABR2_Antibody_013122
Total tissue lysates (15 µg total protein) from rat brain (Lane 1), mouse brain (Lane 2), human brain (Lane 3), and human kidney (Lane 4, negative control) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-GABABR2 recombinant (clone QA19A84) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin (Cat. No. 664803) was used as a loading control at a 1:50000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
  • QA19A84_PURE_GABABR2_Antibody_013122
    Total tissue lysates (15 µg total protein) from rat brain (Lane 1), mouse brain (Lane 2), human brain (Lane 3), and human kidney (Lane 4, negative control) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-GABABR2 recombinant (clone QA19A84) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin (Cat. No. 664803) was used as a loading control at a 1:50000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
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600251 25 µg 104€
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600252 100 µg 259€
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Description

Gamma-aminobutyric acid, B [GABAB] receptors are located in the midbrain and help facilitate inhibitory signaling in the central nervous system. GABAB receptors exist as heterodimers, comprising of two subunits: GABABR1 and GABABR2 with 7 transmembrane domains, neither of which is fully functional when expressed alone. The GABABR1 inhibits neurotransmitter activity via binding γ-aminobutyric acid (GABA), following G protein-coupled pathway activation through interacting with GABABR2. Subsequently, this signaling cascade changes ion channel activity and induces adenylyl cyclase to interfere synaptic responses. A decrease in GABABR2 functional efficacy is implicated in several diseases, such as Rett syndrome and epileptic encephalopathy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Mouse, Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Partial recombinant mouse GABABR2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It is recommended to boil cell or tissue lysates at 70°C for western blot.

Reactivity of clone QA19A84 with human GABABR2 is observed but is weaker than reactivity with mouse or rat GABABR2.

RRID
AB_2910469 (BioLegend Cat. No. 600251)
AB_2910469 (BioLegend Cat. No. 600252)

Antigen Details

Structure
GABABR2 is a 941 amino acid protein with a molecular mass of 106 kD.
Distribution

Membrane

Function
Synaptic Transmission
Interaction
GABABR1
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Antigen References
  1. Yoo Y, et al. 2017. Ann Neurol. 82:466-478.
  2. Duthey B, et al. 2002. J Biol Chem. 277:3236-41.
  3. Filippov A, et al. 2000. J Neurosci. 20:2867-74.
  4. Blein S, et al. 2000. Cell Mol Life Sci. 57:635-50.
Gene ID
9568 View all products for this Gene ID
UniProt
View information about GABABR2 on UniProt.org
Go To Top Version: 1    Revision Date: 01.31.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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