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Purified anti-Gemin 4 Antibody

Pricing & Availability
Clone
17D10 (See other available formats)
Regulatory Status
RUO
Other Names
P97, Gem Nuclear Organelle Associated Protein 4, Component of Gems 4, Gem-Associated Protein 4, HCC-Associated Protein 1, Gemin-4, Gem (Nuclear Organelle) Associated Protein 4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
17D10_PURE_Gemin-4_Antibody_091619.png
Western blot of purified anti-Gemin 4 antibody (clone 17D10). Lane 1: Molecular weight marker; Lane 2: 10 µg of HEK293 cell lysate; Lane 3: 10 µg of HepG2 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Note that expression of Gemin 4 in HEK293 cells is much higher than in HepG2 cells.
  • 17D10_PURE_Gemin-4_Antibody_091619.png
    Western blot of purified anti-Gemin 4 antibody (clone 17D10). Lane 1: Molecular weight marker; Lane 2: 10 µg of HEK293 cell lysate; Lane 3: 10 µg of HepG2 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Note that expression of Gemin 4 in HEK293 cells is much higher than in HepG2 cells.
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869601 25 µg 85€
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869602 100 µg 221€
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Description

Gemin 4 is one of the core components of the survival of motor neurons (SMNs) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Recent studies have shown that one of the three putative nuclear localization signal motifs in Gemin 4 is necessary and sufficient to direct the nuclear import of the other two SMN complex components, Gemin 2 and Gemin 3. This suggests that Gemin 4 plays an essential role in mammalian snRNP biogenesis via facilitating the import of the SMN complex or subunits into the nucleus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Histidine-tagged recombinant protein corresponding to human Gemin 4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)

Battle DJ, et al. 2007. J. Biol Chem. 282(38):27953 (WB)

RRID
AB_2814637 (BioLegend Cat. No. 869601)
AB_2814637 (BioLegend Cat. No. 869602)

Antigen Details

Structure
Gemin 4 is a 1058 amino acid protein with predicted and observed molecular mass of ~120 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and cytosol

Function
Gemin 4 is a component of the SMN macromolecule protein complex.
Interaction
Gemin 4 is a part of the core SMN complex that contains SMN 1-8.
Biology Area
Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Meier ID, et al. 2018. Biol Open. 7(2)
  2. Battle DJ, et al. 2007. J Biol Chem. 282(38):27953
  3. Wang JL, et al. 2004. Biochim Biophys Acta. 1673(1-2):75.
Gene ID
50628 View all products for this Gene ID
UniProt
View information about Gemin 4 on UniProt.org
Go To Top Version: 1    Revision Date: 09.16.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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