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- Clone
- 10E2/HDAC1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
- Previously
-
Covance Catalog# MMS-5071
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of purified anti-HDAC1 antibody (clone 10E2/HDAC1) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Total cell lysates (15 µg protein) from HeLa (lanes marked as 1) and NIH3T3 cells (lanes marked as 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with serial dilutions of purified anti-HDAC1 antibody (clone 10E2/HDAC1) as indicated (1:500, 1 µg/mL; 1:1000, 0.5 µg/mL; 1:2000, 0.25 µg/mL; 1:4000, 0.125 µg/mL). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated goat anti-mouse-IgG secondary antibody for the anti-HDAC1 (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. Non-specific bands can be detected at high antibody concentrations. -
Total cell lysates (10 µg total protein) from HeLa (WT) and HeLa HDAC1 CRISPR/CAS9 knockout (KO) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti- HDAC1 Antibody, clone 10E2/HDAC1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 815102 | 25 µL | 81€ | ||||
| 815101 | 100 µL | 220€ | ||||
HDAC1 (histone deacetylase 1) is a 65 kD member of the Class I histone deacetylase family, which is similar to the yeast RPD3 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC1 deacetylates RELA at residue K310, which results in the inhibition of NF-κB activity. HDAC1 has been suggested to participate in the regulation of eukaryotic gene expression and cell cycle progression. HDAC1-deficient mice were shown to be embryonic lethal, suggesting that HDAC1 plays a crucial role during embryonic development.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC1 protein.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB, KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
- RRID
-
AB_2564789 (BioLegend Cat. No. 815102)
AB_2564789 (BioLegend Cat. No. 815101)
Antigen Details
- Structure
- Expected MW: 60 kD
- Biology Area
- Cell Biology, Neuroscience
- Gene ID
- 3065 View all products for this Gene ID
- UniProt
- View information about HDAC1 on UniProt.org
Related FAQs
Other Formats
View All HDAC1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-HDAC1 | 10E2/HDAC1 | IHC-P,WB,KO/KD-WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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