- Clone
- W15091A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Aryl hydrocarbon receptor nuclear translocator (ARNT), Class E basic helix-loop-helix protein 2 (bHLHe2), Hypoxia-inducible factor 1-beta
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
689602 | 100 µg | 203€ |
Hypoxia-inducible factor 1 beta (HIF1-β), also known as Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), functions mainly as a transcription factor. This protein contains a basic helix-loop-helix domain, two PAS (PER-ARNT-SIM) domains and one PAC (PAS-associated c-terminal) domain. HIF1-β binds to the ligand-bound aryl hydrocarbon (Ah) receptor and helps the complex move from cytosol to the nucleus, where it promotes the expression of xenobiotic metabolism-related genes. HIF1-β is also involved in cellular senescence and HIF-1 transcription factor network. It forms a heterodimer with HIF1-α or EPAS1/HIF2A and transcriptionally regulates the response to hypoxia. There are multiple transcript variants of HIF1-β generated by alternative splicing.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human HIF1-β recombinant protein (517-691 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W15091A does not cross-react with mouse.
- RRID
-
AB_2629788 (BioLegend Cat. No. 689602)
Antigen Details
- Structure
- 789 amino acids with a predicted molecular weight of 86.6 kD. Contains a bHLH (basic helix-loop-helix) domain, a PAC (PAS-associated C-terminal) domain, and two PAS (PER-ARNT-SIM) domains.
- Distribution
-
Nucleus
- Function
- HIF1-β interacts with ligand-bound aryl hydrocarbon receptor (AhR) and acts as a transcriptional co-activator for the genes involved in xenobiotic metabolism.
- Interaction
- Forms a heterodimer with AHR, AHRR, HIF1A, EPAS1/HIF2, and other bHLH proteins. Interacts with TACC3 and NOCA7.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
1. Furue M, et al. 2014. Arch Dermatol. Res. 306:769.
2. Vorrink SU, et al. 2014. Chem. Biol. Interact. 218:82.
3. Mandl M, et al. 2014. Mol. Med. 20:215.
4. Gu C, et al. 2014. Leuk. Res. 37:1750.
5. Wolff M, et al. 2013. Cell Physiol Biochem. 32:849.
6. Nakajima K, et al. 2013. Nat. Commun. 4:2112. - Gene ID
- 405 View all products for this Gene ID
- UniProt
- View information about HIF1-beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HIF1-β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HIF1-β | W15091A | WB |
Compare Data Across All Formats
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Purified anti-HIF1-β
Total cell lysates (15 µg total protein) from THP-1, SH-SY5Y...
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