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Purified anti-HMOX1 Antibody

Pricing & Availability
Clone
W19398C (See other available formats)
Regulatory Status
RUO
Other Names
Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W19398C_PURE_HMOX1_Antibody_WB_1_070721.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-HMOX1 antibody (clone W19398C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 2.0 µg/mL dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • A_W19398C_PURE_HMOX1_Antibody_WB_1_070721.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-HMOX1 antibody (clone W19398C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 2.0 µg/mL dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • B_W19398C_PURE_HMOX1_Antibody_WB_2_070721.png
    Total cell lysates (15 µg total protein) from HaCat cells untreated or stimulated with 0.75 mM CoCl2 for 3-8 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) purified anti-HMOX1 antibody (clone W19398C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 2.0 µg/mL. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • C_W19398C_PURE_HMOX1_ICC_070721.png
    HaCat cells were untreated (panel A) or stimulated with 0.75 mM CoCl2 for 8 hours (panel B). Cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Fixed cells were then intracellularly stained with 5 µg/mL (1:100 dilution) purified anti-HMOX1 antibody (clone W19398C) overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • D_W19398C_PURE_HMOX1_Antibody_IP_070721.png
    Whole cell extracts (250 μg total protein) prepared from A549 cells were immunoprecipitated overnight with 2.5 μg of purified rat IgG2b, k isotype ctrl antibody (Cat. No. 400602) or purified anti-HMOX1 antibody (clone W19398C). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with an HMOX1 antibody other than W19398C. Lane M: Molecular weight marker.
  • E_W19398C_PURE_HMOX1_ICFC_070721.png
    HaCaT cells treated (filled histogram, positive control) or untreated (open histogram, negative control) with 0.75 mM of CoCl2 for 8 hours were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with purified anti-HMOX1 antibody (clone W19398C) followed by PE goat anti-rat IgG antibody (Cat. No. 405406).
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934101 25 µg 95€
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934102 100 µg 235€
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Description

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 - 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100.

In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells.

Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results). 

 

RRID
AB_2894520 (BioLegend Cat. No. 934101)
AB_2894520 (BioLegend Cat. No. 934102)

Antigen Details

Distribution

Cytosol/Endoplasmic reticulum

Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197
2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47
3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.

Gene ID
3162 View all products for this Gene ID
UniProt
View information about HMOX1 on UniProt.org
Go To Top Version: 1    Revision Date: 07.07.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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