- Clone
- W19398C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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934101 | 25 µg | 95€ | ||||
934102 | 100 µg | 235€ |
HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 - 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100.
In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells.
Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results). - RRID
-
AB_2894520 (BioLegend Cat. No. 934101)
AB_2894520 (BioLegend Cat. No. 934102)
Antigen Details
- Distribution
-
Cytosol/Endoplasmic reticulum
- Biology Area
- Cell Biology
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197
2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47
3. Harding H, et al. 2003. Mol Cell. 11(3):619-33. - Gene ID
- 3162 View all products for this Gene ID
- UniProt
- View information about HMOX1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HMOX1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HMOX1 Antibody | W19398C | WB,ICC,ICFC,IP |
PE anti-HMOX1 | W19398C | ICFC |
Alexa Fluor® 647 anti-HMOX1 | W19398C | ICFC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-HMOX1 Antibody
Whole cell extracts (15 µg protein) from the indicated cell ... Total cell lysates (15 µg total protein) from HaCat cells un... HaCat cells were untreated (panel A) or stimulated with 0.75... Whole cell extracts (250 μg total protein) prepared from A54... HaCaT cells treated (filled histogram, positive control) or ... -
PE anti-HMOX1
HeLa cells untreated (low expression control, open histogram... -
Alexa Fluor® 647 anti-HMOX1
HeLa cells untreated (low expression control, open histogram... HeLa cells untreated (panels A and B) or treated with 200 µM...
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