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Purified anti-human C5a/C5a(desArg) Antibody

Pricing & Availability
Clone
C17/5 (See other available formats)
Regulatory Status
RUO
Other Names
C5a, C5a desArg
Isotype
Mouse IgG1, κ
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Product Citations
publications
C17slash5_Pure_020312
  • C17slash5_Pure_020312
Cat # Size Price Quantity Check Availability Save
518201 50 µg 81€
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518202 500 µg 259€
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Description

C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and  is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human C5a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The C17/5 antibody reacts with human C5a and C5a(desArg). C17/5 is specific for a C5 activation-dependent neo-epitope expressed only on C5a and C5a(desArg) but not C5, and has been shown to be an effective inhibitor of C5a.4

ELISA Capture: The purified C17/5 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated G25/2 antibody (Cat. No. 518306) as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Oppermann M, et al. 1991. Kidney Int. 40:939947.
  2. Oppermann M, et al. 1992. Immunopharm. 24:119134.
  3. Elsner J, et al. 1994. Blood 83:3324.
  4. Wittmann M, et al. 1999. J. Immunol. 162: 6763.
Product Citations
  1. Throm AA, et al. 2018. JCI Insight. :3. PubMed
RRID
AB_11123910 (BioLegend Cat. No. 518201)
AB_11123910 (BioLegend Cat. No. 518202)

Antigen Details

Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lundwall AB, et al. 1985. J. Biol. Chem. 260:2108.
2. Fernandez HN, et al. 1978. J. Biol. Chem. 253:6955.
3. Tack BF, et al. 1979. Biochemistry 18:1490.

Gene ID
727 View all products for this Gene ID
UniProt
View information about C5a/C5adesArg/C5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10.17.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human C5a/C5a(desArg)

    C17slash5_Pure_020312
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