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Purified anti-human CD151 (PETA-3) Antibody

Pricing & Availability
Clone
50-6 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet-endothelial tetraspan antigen 3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
50-6_APC_092210
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
  • 50-6_APC_092210
    Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
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350402 100 µg 100€
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Description

CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains, and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, and vascular endothelium. On the cell surface, PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1, and α6β4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human epidermoid carcinoma cell line HEp-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo inhibition of metastasis of HEp-3 cells1, Western blotting1, immunocytochemical staining of HEp-3 cells1, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Testa JE, et al. 1999. Cancer Res. 59:3812. (Block, ICC, WB)
RRID
AB_10661898 (BioLegend Cat. No. 350402)

Antigen Details

Structure
Member of the tetraspanin family (TM4SF), 29 kD protein, consist of two extracellular loop, four transmembrane domains and two cytoplasmic tails
Distribution

Immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium

Function
Cell adhesion, migration
Interaction
CD9, CD181, integrins α3β1, α5β1 and α6β4
Cell Type
Epithelial cells, Hematopoietic stem and progenitors, Megakaryocytes, Mesenchymal Stem Cells, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zöller M. 2009. Nat. Rev. Cancer 9:40.
2. Yañez-Mó M, et al. 2008. Blood 112:3217.
3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.

Gene ID
977 View all products for this Gene ID
UniProt
View information about CD151 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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