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Purified anti-human CD164 Antibody

Pricing & Availability
Clone
67D2 (See other available formats)
Regulatory Status
RUO
Other Names
Sialomucin CD164, MUC-24, multi-glycosylated core protein 24, MGC-24
Isotype
Mouse IgG1, κ
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Product Citations
publications
67D2_Pure_011408
Human myeloid cell line KG1a stained with purified 67D2, followed by anti-mouse IgG FITC
  • 67D2_Pure_011408
    Human myeloid cell line KG1a stained with purified 67D2, followed by anti-mouse IgG FITC
  • 67D2_Purified_CD164_Antibody_ICC_011421
    BT474 breast cancer cell line was stained with anti-human CD164, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
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324802 100 µg 95€
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Description

The 67D2 monoclonal antibody recognizes human CD164 also known as sialomucin CD164, MUC-24, and multi-glycosylated core protein 24. CD164 is a single pass transmembrane protein with short cytoplasmic tail that is highly N- and O-glycosylated. This protein contains sialic acid and a Ser-Gly motif that may serve as an attachment for a glycosaminoglycan side chain. Three splice variants of CD164 have been reported with apparent molecular weights ranging between 80-100 kD. CD164 is expressed in bone marrow, bone marrow stromal cells, and CD34+ hematopoietic cells myeloid and erythroid progenitors; and activated basophils. Expression has also been reported on a variety of carcinomas and leukemic cells and in the small intestine, colon, lung, and thyroid. CD164 plays a role in cell adhesion and proliferation and acts as a negative signaling molecule for hematopoietic progenitor cells. CD164 has also been reported to be involved in myogenic differentiation and cancer metastasis. The 67D2 antibody has been shown to be useful for the flow cytometric detection of human CD164, Western blotting under non-reducing conditions (detects an 80-100 kD protein as well as a high molecular weight aggregate of approximately 220 kD), and immunofluorescence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T-47D breast carcinoma line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2,3 under non-reducing conditions (detects an 80-100 kD protein as well as a high molecular weight aggregate of approximately 220 kD), and immunofluorescence3.

Application References

(PubMed link indicates BioLegend citation)
  1. Watt SM, et al. 1998. Blood 92:849.
  2. Watt SM, et al. 2000. Blood 95:3113.
  3. Doyonnas R, et al. 2000. J. Immunol. 165:840.
  4. Vogel W, et al. 2002. Haematologica 88:126.
Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  2. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  3. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
RRID
AB_756020 (BioLegend Cat. No. 324802)

Antigen Details

Structure
Single pass transmembrane protein with short cytoplasmic tail. Highly N- and O-glycosylated, contains sialic acid and a Ser-Gly motif that may serve as an attachment for a glycosaminoglycan side chain. Three splice variants have been reported with apparen
Distribution

Expressed in bone marrow, bone marrow stromal cells, and CD34+ hematopoietic cells myeloid and erythroid progenitors; activated basophils. Expressed on a variety of carcinomas and leukemic cells. Also expressed in the small intestine, colon, l

Function
Plays a role in cell adhesion and proliferation, negative signaling molecule for hematopoietic progenitor cells. Has also been reported to be involved in myogenic differentiation and cancer metastasis
Cell Type
Basophils, Hematopoietic stem and progenitors, Leukemia
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zannettino ACW, et al. 1998. Blood 92:2613.
2. Havens AM, et al. 2006. BMC Cancer 6:195.
3. Lee YN, et al. 2001. Mol. Cell. Biol. 21:7696.

Gene ID
8763 View all products for this Gene ID
UniProt
View information about CD164 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08.30.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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