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Purified anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_HIB19_Purified_061807
Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC
  • 1_HIB19_Purified_061807
    Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC
  • 2_HIB19_PURE_CD19_Antibody_IHC-F_091018
    Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD19 antibody (clone HIB19) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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302201 25 µg 24€
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302202 100 µg 48€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
PG - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  3. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  4. Donnadieu E, et al. 2020. J Immunol. 205:1248. PubMed
  5. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  6. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  7. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  8. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  9. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  10. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
  11. Weinhage T, et al. 2020. J Immunol. 205:56. PubMed
  12. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  13. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  14. Walker J, et al. 2009. J Immunol. 182:1548. PubMed
  15. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  16. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  17. Pugh J, et al. 2021. PeerJ. 9:e12258. PubMed
  18. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  19. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  20. Lartey S, et al. 2020. J Infect Dis. 221:21. PubMed
  21. Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed
  22. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  23. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  24. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  25. Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
  26. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  27. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  28. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  29. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  30. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  31. Smillie CS et al. 2019. Cell. 178(3):714-730 . PubMed
  32. Ferdosi SR, et al. 2019. Nat Commun. 1.695833333. PubMed
  33. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  34. Cerignoli F, et al. 2018. PLoS One. 13:e0193498. PubMed
  35. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  36. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  37. Johnston HE, et al. 2018. Mol Cell Proteomics. 1.247222222. PubMed
  38. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  39. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  40. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  41. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  42. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  43. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  44. Renand A, et al. 2018. J Allergy Clin Immunol. 141:1750. PubMed
  45. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  46. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  47. Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed
  48. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  49. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  50. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  51. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  52. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  53. Han SS, et al. 2018. Oncotarget. 9:3292. PubMed
  54. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  55. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
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  57. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  58. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  59. Crome SQ, et al. 2017. Nat Med. 1.213888889. PubMed
  60. Afzali B, et al. 2017. Nat Immunol. 18:813. PubMed
  61. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  62. Vanshylla K, et al. 2018. Eur J Immunol. 48:441. PubMed
  63. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  64. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  65. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  66. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  67. Martin E, et al. 2020. JCI Insight. :5. PubMed
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RRID
AB_314231 (BioLegend Cat. No. 302201)
AB_314231 (BioLegend Cat. No. 302202)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
Spark UV™ 387 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Alexa Fluor® 660 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV395™ anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
Go To Top Version: 6    Revision Date: 01.06.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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