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- Clone
- SHM72 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MFI2, MAP97, MTF1, Melanotransferrin, Membrane-bound transferrin-like protein
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human malignant melanoma cell line, A375, was stained with purified CD228 (clone SHM72, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 363101 | 100 µg | 212€ | ||||
CD228 is a cell-surface sialoglycoprotein found on melanoma cells that consists of a cell membrane, lipid-anchor and GPI-anchor domains. It is involved in iron uptake and shares sequence similarity and iron-binding properties that are found in members of the transferrin superfamily. CD228 is internalized before being recycled back to the cell membrane and is also capable of binding zinc.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2564116 (BioLegend Cat. No. 363101)
Antigen Details
- Structure
- Cell membrane, lipid-anchor, GPI-anchor, 80 kD
- Distribution
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Melanoma cells
- Function
- Involved in iron uptake
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Farnaud S, et al. 2008. Int. J. Biochem. Cell Biol. 40:2739.
2. Sekyere EO, et al. 2005. Biochim. Biophys. Acta. 1722:131.
3. Dunn LL, et al. 2006. Carcinogenesis. 27:2157.
4. Suryo Rahmanto Y, et al. 2007. Carcinogenesis. 28:2172. - Gene ID
- 4241 View all products for this Gene ID
- UniProt
- View information about CD228 on UniProt.org
Other Formats
View All CD228 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD228 (MFI2, MTF1) | SHM72 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD228 (MFI2, MTF1)
Human malignant melanoma cell line, A375, was stained with p...
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