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- Clone
- MIH4.Rec (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD1, P:D-1, PDCD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified anti-human CD279 (PD-1) (clone MIH4.Rec) (left) or purified mouse IgG1, κ isotype control (right) followed by goat anti-mouse IgG PE. Cells were then stained with anti-human CD3 (clone UCHT1) APC. -
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified anti-human CD279 (PD-1) (clone MIH4.Rec) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by goat anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 335703 | 100 µg | 90€ | ||||
CD279, also known as Programmed cell Death 1 (PD-1), is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 and are members of the B7 gene family. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
MIH4.rec clone does not affect the binding of clones NAT105, EH12.2H7, A17188A, and A17188B on target cells.
Antigen Details
- Structure
- 55 kD, immunoglobulin superfamily
- Distribution
-
Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells
- Function
- Signaling, co-stimulation (co-inhibition)
- Interaction
- SHP-1 and SHP-2.
- Ligand/Receptor
- PD-L1 (CD274) and PD-L2 (CD273)
- Cell Type
- Lymphocytes, T cells
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
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- Francisco LM, et al. 2010. Immunological Rev. 236:219.
- Gene ID
- 5133 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
Related FAQs
Other Formats
View All CD279 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD279 (PD-1) Recombinant Antibody | MIH4.Rec | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD279 (PD-1) Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... 
PHA-stimulated (3 days) human peripheral blood lymphocytes w...
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