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Purified anti-human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
A18180C (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, cadherin-1, UVO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A18180C_Purified_CD324_Antibody_2_101524-2
Human peripheral blood mononuclear cells were stained with purified anti-human CD324 (E-Cadherin) (clone A18180C) (left) or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then stained with anti-human CD123 (clone 6H6) APC. Data shown was gated on lymphocytes and monocytes.
  • A. A18180C_Purified_CD324_Antibody_2_101524-2
    Human peripheral blood mononuclear cells were stained with purified anti-human CD324 (E-Cadherin) (clone A18180C) (left) or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then stained with anti-human CD123 (clone 6H6) APC. Data shown was gated on lymphocytes and monocytes.
  • B. A18180C_Purified_CD324_Antibody_1_101524
    Human breast cancer cells, MCF-7, were stained with purified anti-human CD324 (E-Cadherin) (clone A18180C) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • C. A18180C_Purified_CD324_Antibody_3_101524
    IHC staining of purified anti-human CD324 (E-Cadherin) (clone A18180C) on formalin-fixed paraffin-embedded human kidney tissue. After antigen retrieval using 1X Tris Buffered Saline with Tween (Cat. No. 925501), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of purified anti-human CD324 (E-Cadherin) (clone A18180C), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 200 µm
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Description

The A18180C antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II, as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The A18180C antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein aa#155-707
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. Predicted molecular weight approximately 100 kD.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cell, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase 8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others
Ligand/Receptor
αEβ7 integrin
Cell Type
Embryonic Stem Cells, Epithelial cells, Erythrocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Overduin M, et al. 1995. Science. 267:386-9.
  2. Boggon TJ, et al. 2002. Science. 296:1308-13.
  3. Berx G, et al. 1995. EMBO J. 14:6107-15.
  4. Perl AK, et al. 1998. Nature. 392:190-3.
Gene ID
999 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org
Go To Top Version: 1    Revision Date: 10.15.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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