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Purified anti-human CD99 Antibody

Pricing & Availability
Clone
3B2/TA8 (See other available formats)
Regulatory Status
RUO
Other Names
CD99, MIC2, HBA71, MSK5X, E2 antigen
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
3B2slashTA8_PURE_CD99_Antibody_031816
Human peripheral blood lymphocytes were stained with purified CD99 (clone 3B2/TA8, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 3B2slashTA8_PURE_CD99_Antibody_031816
    Human peripheral blood lymphocytes were stained with purified CD99 (clone 3B2/TA8, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 3B2slashTA8_PURE_CD99_Antibody_IHC-P_111519
    Human paraffin-embedded pancreas tissue slice was stained with purified anti-human CD99 (clone 3B2/TA8) antibody overnight followed by Alexa Fluor® 488 goat anti-mouse secondary antibody (green). The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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371302 100 µg 95€
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Description

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human thymus
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Waclavicek M, et al. 1998. J. Immunol. 161:4671.
  2. Pickl W, et al. 2001. J. Virol. 75:7175.
RRID
AB_2572177 (BioLegend Cat. No. 371302)

Antigen Details

Structure
Surface glycoprotein, type I transmembrane protein, 32kD.
Distribution

T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.

Function
Leukocyte migration, T-cell adhesion, T-cell death- caspase independent, and spontaneous rosette formation with erythrocytes.
Interaction
Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
Cell Type
Leukemia, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gelin C, et al. 1989. EMBO. 8:3253.
2. Goodfellow PJ, et al. 1986. Science 234:740.
3. Pettersen RD, et al. 2001. J. Immunol. 166:4931.

Gene ID
4267 View all products for this Gene ID
UniProt
View information about CD99 on UniProt.org
Go To Top Version: 2    Revision Date: 02.10.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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