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Purified anti-human EGFRvIII Recombinant Antibody

Pricing & Availability
Clone
DH8.3.Rec (See other available formats)
Regulatory Status
RUO
Other Names
EGFRv3
Isotype
Mouse IgG1, κ
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Product Citations
publications
DH83Rec_PURE_EGFRvIII_FC_100924
Human glioblastoma cell line, DKMG/EGFRvIII, was stained with purified anti-human EGFRvIII recombinant (clone DH8.3.Rec) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • DH83Rec_PURE_EGFRvIII_FC_100924
    Human glioblastoma cell line, DKMG/EGFRvIII, was stained with purified anti-human EGFRvIII recombinant (clone DH8.3.Rec) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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335612 100 µg 349€
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Description

Epidermal growth factor receptor variant III (EGFRvIII) arises from an in-frame deletion of exons 2 to 7 in the extracellular region of EGFR, resulting in a truncated receptor that is constitutively active. This mutation is cancer-specific and is present in approximately 50% of glioblastoma tumors. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Peptide LEEKKGNYVVTDHC conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Deletion of exons 2-7 of the EGFR gene. Molecular mass of approximately 145 kD.
Distribution

Present in approximately 50% of glioblastoma tumors

Function
Pro-tumorigenic (promotes tumor growth, survival, invasion, stemness, metabolism, and angiogenesis).
Biology Area
Cancer Biomarkers
Antigen References
  1. Hui G, et al. 2013. FEBS Journal. 280:21.
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFRvIII on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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