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Purified anti-human erbB3/HER-3 Antibody

Pricing & Availability
Clone
1B4C3 (See other available formats)
Regulatory Status
RUO
Other Names
erbB-3, erbB3, HER-3, HER3
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
a-1B4C3_Pure_111407
Human breast cancer cell line MCF-7 stained with purified 1B4C3, followed by biotinylated anti-mouse IgG and Sav-PE
  • a-1B4C3_Pure_111407
    Human breast cancer cell line MCF-7 stained with purified 1B4C3, followed by biotinylated anti-mouse IgG and Sav-PE
  • b-1B4C3_IF_072712
    BT474 breast cancer cells were stained with anti-HER3 (clone 1B4C3) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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324702 100 µg 165€
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Description

The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3/HER3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with human erbB3/HER3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Jenke R, et al. 2020. Int J Mol Sci. 22:. PubMed
  2. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  3. Rau A, et al. 2021. MAbs. 1902034:13. PubMed
RRID
AB_756156 (BioLegend Cat. No. 324702)

Antigen Details

Structure
Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD
Distribution

Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in b

Function
Induces extensive signaling cascade that can regulate growth and possibly differentiation
Ligand/Receptor
Heregulin
Modification
Extensively phosphorylated T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, Y1328
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Carraway KL, et al. 1994. J. Biol. Chem. 269:14303.
2. Kraus MH, et al. 1989. Proc. Natl. Acad. Sci. USA 86:9193.
3. Chan SD, et al. 1995. J. Biol. Chem. 270:22608.
4. Tanner B, et al. 2006. J. Clin. Oncol. (Epub Aug. 8 2006)

Gene ID
2065 View all products for this Gene ID
UniProt
View information about erbB3/HER-3 on UniProt.org
Go To Top Version: 2    Revision Date: 08.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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