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Purified anti-human HLA-F Antibody

Pricing & Availability
Clone
3D11/HLA-F (See other available formats)
Regulatory Status
RUO
Other Names
HLAF, CDA12, HLA-5.4, HLA-CDA12
Isotype
Mouse IgG1, κ
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Product Citations
publications
3D11slashHLA-F_PURE_HLA-F_Antibody_1_022417
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD20 Pacific Blue™ and purified HLA-F (clone 3D11/HLA-F, top) or purified mouse IgG1, κ isotype control (bottom), followed by PE anti-mouse IgG1 secondary.
  • 3D11slashHLA-F_PURE_HLA-F_Antibody_1_022417
    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD20 Pacific Blue™ and purified HLA-F (clone 3D11/HLA-F, top) or purified mouse IgG1, κ isotype control (bottom), followed by PE anti-mouse IgG1 secondary.
  • 3D11slashHLA-F_PURE_HLA-F_Antibody_2_022417
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373202 100 µg 165€
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Description

A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Complex mixed with IFA, complex without adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Direct ELISA, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ishitani A, et al. 2003. J. Immunol. 171:1376.
RRID
AB_2650870 (BioLegend Cat. No. 373202)

Antigen Details

Structure
42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
Distribution

B, NK, monocyte and T cells.

Function
MHC-I OC stabilization, antigen cross presentation.
Ligand/Receptor
KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References
  1. Goodridge JP, et al. 2013. J. Immunol. 191:1567.
  2. Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552.
  3. Burian A,et al. 2016. PLoS One 11:e0163297.
  4. Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
Gene ID
3134 View all products for this Gene ID
UniProt
View information about HLA-F on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03.08.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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