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Purified anti-human Ig light chain λ Antibody

Pricing & Availability
Clone
MHL-38 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain lambda
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MHL-38_Pure_020708
Over night cultured human peripheral blood lymphocytes stained with purified MHL-38, followed by anti-mouse IgG FITC
  • MHL-38_Pure_020708
    Over night cultured human peripheral blood lymphocytes stained with purified MHL-38, followed by anti-mouse IgG FITC
  • MHL-38_PURE_IgLightChainLambda_Antibody_052022
    IHC staining using purified anti-human Ig light chain λ antibody (clone MHL-38) on formalin-fixed paraffin-embedded human tonsil tissue. The tissue was incubated with 5 μg/mL of anti-human Ig light chain λ antibody and co-stained with 5 μg/mL of pan-B cell marker CD19 overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (yellow) (Cat. No. 405322) and 2.5 μg/mL of Alexa Fluor® 594 goat anti-rabbit IgG antibody (red) (Cat. No. 406414) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM.
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316602 100 µg 81€
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Description

The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (κ) or heavy chains. The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 in 100 µl staining volume or 100 µl of whole blood. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).

Product Citations
  1. Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
RRID
AB_493621 (BioLegend Cat. No. 316602)

Antigen Details

Structure
Ig family
Distribution

B cells

Cell Type
B cells
Biology Area
Immunology
Gene ID
3535 View all products for this Gene ID
UniProt
View information about Ig light chain lambda on UniProt.org
Go To Top Version: 4    Revision Date: 05.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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