Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Clone
- C8.3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 501702 | 500 µg | 184€ | ||||
The C8.3 antibody reacts with human interleukin-12 and interleukin-23 (IL-12/IL-23) p40 monomer or homodimer. This antibody does not react with either the p35 subunit of IL-12 or the p19 subunit of IL-23, or the p40 protein when it is heterodimerized with p35 (the IL-12 p70) or p19 (IL-23 p60). The C8.3 antibody cannot neutralize the antagonistic activity of IL-12 p40.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- COS-expressed p40 subunit of human IL-12
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-12 (p40) recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.
- Application Notes
-
ELISA or ELISPOT Capture: The purified C8.3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C8.6 antibody (Cat. No. 508802/508805) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. This assay detects specifically human IL-12/IL-23 p40 protein, without interference by IL-12 p70 or IL-23 p19 proteins.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended. -
Application References
(PubMed link indicates BioLegend citation) -
- D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
- D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
- Product Citations
-
- RRID
-
AB_2255186 (BioLegend Cat. No. 501702)
Antigen Details
- Structure
- Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
- Bioactivity
- IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by hu
- Cell Sources
- Dendritic cells, monocytes/macrophages, B cells, T cells
- Cell Targets
- T cells, NK cells, PBL, mature dendritic cells
- Receptors
- IL-12Rβ1 binds p40 from both IL-12 and IL-23; dimeric with IL-12Rβ2 binds p35; IL-23 specific receptor subunit, IL-23R binds p19
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992. Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993. Immunol. Today. 14:335.
5. Oppmann B, et al. 2000. Immunity. 13:715.
6. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002. J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002. J. Immunol. 168:5448.
9. Lankford CS, et al. 2003. J. Leukoc. Biol. 73:49. - Regulation
- Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function
- Gene ID
- 3593 View all products for this Gene ID
- UniProt
- View information about IL-12/IL-23 p40 on UniProt.org
Related FAQs
Other Formats
View All IL-12/IL-23 p40 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-12/IL-23 p40 (monomer, homodimer) | C8.3 | ELISA Capture |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human IL-12/IL-23 p40 (monomer, homodimer)
Follow Us