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Purified anti-human Lymphotoxin beta receptor (LT-βR) Antibody

Pricing & Availability
Clone
31G4D8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphotoxin beta receptor, Tumor necrosis factor receptor superfamily member 3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
31G4D8_Pure_030308
Human peripheral blood monocytes stained with purified 31G4D8, followed by biotinylated anti-mouse IgG and Sav-PE
  • 31G4D8_Pure_030308
    Human peripheral blood monocytes stained with purified 31G4D8, followed by biotinylated anti-mouse IgG and Sav-PE
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322002 100 µg 113€
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Description

LT-βR, also known as lymphotoxin beta receptor and tumor necrosis factor receptor superfamily member 3 is a 47 kD transmembrane protein containing 3 TNF receptor domains. The LT-βR is a receptor for the heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT). LT-βR is highly expressed in the lung, liver, and kidney and moderately expressed expressed in the heart and testes. LT-βR is weakly expressed in the brain, thymus, spleen, and lymph nodes. The cytoplasmic tail of LT-βR interacts with TRAF3, TRAF4, and TRAF5 as well as the hepatitis C virus core protein. Ligand binding to LT-βR has been shown to induce apoptosis (via TRAF3 and TRAF5) and play a role in the development of lymphoid organs. The 31G4D8 antibody has been shown to be useful for flow cytometry, Western blot, immunoprepicitation and and ELISA (detection antibody). In addition, the 31G4D8 antibody is an agonistic antibody for the LT-βR capable of inducing apoptosis (with IFN-γ treatment).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LT-βR Fc protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, ELISA detection1, induction of apoptosis2. The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322003/322004).

Application References

(PubMed link indicates BioLegend citation)
  1. Developer's communication
  2. Chen M-C, et al. 2003. J. Biol. Chem. 278:16073.
Product Citations
  1. Dai Z, et al. 2016. J Immunol. 1089:197. PubMed
RRID
AB_2139071 (BioLegend Cat. No. 322002)

Antigen Details

Structure
Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD
Distribution

Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes.

Function
Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs
Interaction
Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein
Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Crowe PD, et al. 1994 Science 264:707.
Gene ID
4055 View all products for this Gene ID
UniProt
View information about LT-betaR on UniProt.org
Go To Top Version: 2    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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