Loading...

Purified anti-human LYPD3 Antibody

Pricing & Availability
Clone
11A1 (See other available formats)
Regulatory Status
RUO
Other Names
C4.4A, GPI-Anchored Metastasis-Associated Protein C4.4A Homolog, LY6/PLAUR Domain Containing 3
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
11A1_PURE_LYPD3_Antibody_1_022522
Human paraffin-embedded skin tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human LYPD3 (clone 11A1) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 11A1_PURE_LYPD3_Antibody_1_022522
    Human paraffin-embedded skin tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human LYPD3 (clone 11A1) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 11A1_PURE_LYPD3_Antibody_2_031022
    MCF-7 cells were stained with purified anti-human LYPD3 (clone 11A1) (filled histogram) or purified mouse IgG2b, κ isotype control (clone MPC-11) (open histogram) followed by PE anti-mouse IgG.
Compare all formats
Cat # Size Price Quantity Check Availability Save
364502 100 µg 292€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

C4.4A, also known as Ly6/PLAUR domain containing 3 (LYPD-3), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR). C4.4A is also expressed in the placenta, skin and urothelium and can be found in suprabrasal layers of stratified squamous epithelium and is upregulated on migrating keratinocytes during wound healing. Human C4.4A mRNA has been detected in cancer cell lines, including melanoma, breast, bladder, and renal cell carcinoma, as well as in tumor tissue samples from malignant melanoma, colorectal cancer (CRC), breast cancer, lung carcinoma, and urothelial tumors. Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922576 (BioLegend Cat. No. 364502)

Antigen Details

Distribution

Placenta, skin, urothelium, keratinocytes

Function
Interacting selectively and non-covalently with laminins, glycoproteins that are major constituents of the basement membrane of cells
Interaction
LGALS3 (By similarity). AGR2 and AGR3
Ligand/Receptor
Laminin-1 and laminin-5
Biology Area
Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Smith BA, et al. 2001. Cancer Res. 61:1678-85.
  2. Würfle J, et al. 2001. Gene. 262:35-41.
Gene ID
27076 View all products for this Gene ID
UniProt
View information about LYPD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account