- Clone
- MAb11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
502901 | 50 µg | 67€ | ||||
502902 | 500 µg | 188€ |
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- E. coli-expressed, recombinant human TNF-α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA - Quality tested
CyTOF® - Verified
FC, ICFC, ICC, IHC, Neut - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25 - 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 4 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.
Flow Cytometry3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections4 and acetone-fixed frozen tissue sections8, immunocytochemistry7, and immunofluorescence9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.
Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).
The Purified MAb1 antibody is useful in neutralization2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.
Clone MAb11 cross-reacts to Cat11 -
Application References
(PubMed link indicates BioLegend citation) -
- Rathjen D, et al. 1991. Mol. Immunol. 28:79. (Neut)
- Ablamunits V, et al. 2010. Eur. J. Immunol. 40:2891. (Neut)
- Enr quez J, et al. 2002. Adv. Perit. Dial. 18:177. (ICFC)
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
- Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
- Andersson U, et al. 2000. J. Exp. Med. 192:565. (ICC)
- Moormann AM, et al. 1999. J. Infect. Dis. 180:1987. (IHC)
- Zhao XJ, et al. 2003. J. Immunol. 170:2923. (IF)
- Rieger R, et al. 2009. Cancer Gene Ther. 1:53-64. (FC)
- Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC)
- Product Citations
-
- RRID
-
AB_315253 (BioLegend Cat. No. 502901)
AB_315253 (BioLegend Cat. No. 502902)
Antigen Details
- Structure
- TNF superfamily; dimer/trimer; 17 kD (Mammalian)
- Bioactivity
- Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; chemoattractant
- Cell Sources
- Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Cell Targets
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
- Receptors
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
- Cell Type
- Neutrophils, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415. - Regulation
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
- Gene ID
- 7124 View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human TNF-α
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Biotin anti-human TNF-α
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FITC anti-human TNF-α
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PE anti-human TNF-α
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Purified anti-human TNF-α
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Alexa Fluor® 488 anti-human TNF-α
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Alexa Fluor® 647 anti-human TNF-α
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Alexa Fluor® 700 anti-human TNF-α
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Pacific Blue™ anti-human TNF-α
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PerCP/Cyanine5.5 anti-human TNF-α
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PE/Cyanine7 anti-human TNF-α
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Brilliant Violet 421™ anti-human TNF-α
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Brilliant Violet 605™ anti-human TNF-α
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Brilliant Violet 650™ anti-human TNF-α
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Brilliant Violet 711™ anti-human TNF-α
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APC/Cyanine7 anti-human TNF-α
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Purified anti-human TNF-α (Maxpar® Ready)
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PE/Dazzle™ 594 anti-human TNF-α
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Brilliant Violet 785™ anti-human TNF-α
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Brilliant Violet 510™ anti-human TNF-α
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PerCP anti-human TNF-α
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PE anti-human TNF-α
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Spark Blue™ 515 anti-human TNF-α
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Spark UV™ 387 anti-human TNF-α
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TotalSeq™-B0945 anti-human TNF-α
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Brilliant Violet 750™ anti-human TNF-α
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APC/Fire™ 810 anti-human TNF-α
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GMP PE anti-human TNF-α
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