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Purified anti-human TRA-1-60-R Antibody

Pricing & Availability
Clone
TRA-1-60-R (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin, TRA-1
Isotype
Mouse IgM, κ
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Product Citations
publications
Tra-1-60_Pure_090808.jpg
NCCIT (human teratocarcinoma cell line) stained with purified TRA-1-60-R (red line) or purified mouse IgM, κ isotype control (green line) followed by anti-mouse IgM.
  • Tra-1-60_Pure_090808.jpg
    NCCIT (human teratocarcinoma cell line) stained with purified TRA-1-60-R (red line) or purified mouse IgM, κ isotype control (green line) followed by anti-mouse IgM.
  • TRA-1-60_Purified_WB_052314.jpg
    Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2) and Hela (lane 3) cells using anti-TRA-1-60-R antibody (TRA-1-60-R). GAPDH (poly6314) was used a loading control.
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330602 100 µg 216€
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Description

TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R).  The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity.  Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonal carinoma cell line 2102Ep cl.2A6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Verified
ICC, IP, RIA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyoshi N, et. al. 2010. P. Natl. Acad. Sci. USA 107:40. (IF)
  2. Kuai XL, et al. 2009. Differentiation 77:229. (WB)
  3. King F, et al. 2009. Stem Cells Dev. 18:1441. (FC)
Product Citations
  1. Ho V, et al. 2021. Methods Mol Biol. 2436:67. PubMed
  2. Raad S, et al. 2022. Stem Cell Res. 60:102711. PubMed
  3. Schmid B, et al. 2021. Stem Cell Research. 52:102240. PubMed
  4. Hey CAB et al. 2018. Stem Cell Res. 33:46-50 . PubMed
  5. Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed
  6. Hey CAB et al. 2018. Stem cell research. 31:235-239 . PubMed
  7. Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
  8. Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
  9. Gerace D, et al. 2021. Stem Cell Research. 53:102371. PubMed
  10. Lang C et al. 2019. Cell stem cell. 24(1):93-106 . PubMed
  11. Hey CAB, et al. 2020. Stem Cell Research. 41:101594.. PubMed
RRID
AB_1186144 (BioLegend Cat. No. 330602)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Gene ID
5420 View all products for this Gene ID
UniProt
View information about TRA-1-60-R on UniProt.org
Go To Top Version: 2    Revision Date: 05.23.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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