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Purified anti-Lamin B1 Antibody

Pricing & Availability
Clone
5G8-D3-H7 (See other available formats)
Regulatory Status
RUO
Other Names
LMN2, LMNB, ADLD, LMN
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
5G8-D3-H7_PURE_Lamin-B1_Antibody_1_102319
Western blot of purified anti-Lamin B1 antibody (clone 5G8-D3-H7). Lane 1: Molecular weight marker; Lane 2: 30 µg of human cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 5 µg of HeLa cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_1_102319
    Western blot of purified anti-Lamin B1 antibody (clone 5G8-D3-H7). Lane 1: Molecular weight marker; Lane 2: 30 µg of human cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 5 µg of HeLa cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_2_102319
    IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_3_102319
    IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_4_102319
    IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_5_102319
    IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by 2.5 µg/mL Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5G8-D3-H7_PURE_Lamin-B1_Antibody_6_102319
    IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by 2.5 µg/mL Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
869801 25 µg 85€
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869802 100 µg 221€
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Description

Lamin B1 is a type V intermediate filament and an integral component of the nuclear lamina, a meshwork of proteins located inside the nuclear membrane. There are 3 major types of nuclear lamin proteins: lamin A/C, lamin B1 and lamin B2. Lamin A and C are the alternative splicing products of LMNA gene, whereas lamin B1 and lamin B2 are encoded by genes LMNB1 and LMNB2, respectively. Lamins are crucial for maintaining the nuclear structure. They are also important for regulating nuclear functions and activities, including DNA replication and transcription, nuclear and chromatin organization, cell cycle regulation, cell development and differentiation, nuclear migration, and apoptosis. In the brain, lamin B1 levels modulate brain development. Loss of lamin B1 in mouse adversely impairs embryonic brain development and corticogenesis. In addition, duplications of LMNB1 gene causes autosome-dominant leukodystrophy, an adult-onset demyelinating disorder characterized by symmetrical widespread myelin loss in the central nervous system with a phenotype similar to chronic progressive multiple sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Lamin B1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Strine MS, et al. 2023. PLoS Biol. 21:e3002097. PubMed
RRID
AB_2820181 (BioLegend Cat. No. 869801)
AB_2820181 (BioLegend Cat. No. 869802)

Antigen Details

Structure
Human lamin B1 is a 586 amino acid protein with a predicted molecular mass of 66 kD.
Distribution

Tissue distribution: Ubiquitously expressed
Cellular distribution: Nucleus

Function
Lamin B1 is a component of the nuclear lamina, providing a framework for nuclear envelope.
Interaction
Lamin B1 interacts with lamin-associated peptides IA, IB, 2, and SPAG4 and SEPT12.
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Cell Structure, Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Nuclear Markers
Antigen References
  1. Padiath QS. et al. 2006. Nat Genet. 38(10):1114-23.
  2. Camps J. et al. 2015. Nucleus. 6(1):8-14.
  3. Mahajani S. et al. 2017. Sci Rep. 7(1):4897.
Gene ID
4001 View all products for this Gene ID
UniProt
View information about Lamin B1 on UniProt.org
Go To Top Version: 1    Revision Date: 10.23.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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