- Clone
- W17155A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphoid enhancer-binding factor 1, TCF1α, TCF10
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
616001 | 25 µg | 81€ | ||||
616002 | 100 µg | 203€ |
Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
W17155A recognizes multiple isoforms of LEF1.
When tested for ICC, W17155A failed to stain PFA-fixed Jurkat cells permeabilized with methanol. We recommend Triton X-100 permeabilization. - RRID
-
AB_2814460 (BioLegend Cat. No. 616001)
AB_2814460 (BioLegend Cat. No. 616002)
Antigen Details
- Structure
- LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
- Distribution
-
Lymphoid tissue enriched/Nuclear localization
- Function
- Canonical Wnt signaling
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, TCRs
- Antigen References
-
1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184.
2. Waterman ML, et al. 1991. Genes Dev. 5:656.
3. Hovanes K, et al. 2001. Nat Genet. 28:53.
4. Bruhn L, et al. 1997. Genes Dev. 11:640.
5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949.
6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515. - Gene ID
- 51176 View all products for this Gene ID
- UniProt
- View information about LEF1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All LEF1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-LEF1 | W17155A | WB,ICC,IP |
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