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Purified anti-MART-1 Antibody (Previously Covance catalog# SIG-38160)

Pricing & Availability
Clone
M2-7C10 (See other available formats)
Regulatory Status
RUO
Other Names
MLANA, Melanoma antigen recognized by T-cells 1, antigen LB39-AA, antigen SK29-AA, protein Melan-A
Previously
Signet Catalog# 8160-01
Signet Catalog# 8160-26 L2 Predilute
Covance Catalog# SIG-38160
Isotype
Mouse IgG2b, κ
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Product Citations
publications
M2-7C10_PURE_MART-1_Antibody_1_051618
IHC staining of purified anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. Following Retrieve-All Antigen Unmasking System 3: Acidic, 10X, (Cat. No. 927801), the primary antibody was tested at 1:100 dilution overnight at 4°C, followed by incubation with Alexa Fluor® 594 anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • M2-7C10_PURE_MART-1_Antibody_1_051618
    IHC staining of purified anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. Following Retrieve-All Antigen Unmasking System 3: Acidic, 10X, (Cat. No. 927801), the primary antibody was tested at 1:100 dilution overnight at 4°C, followed by incubation with Alexa Fluor® 594 anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • M2-7C10_PURE_MART-1_Antibody_2_051618
    IHC staining of purified anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. Following Retrieve-All Antigen Unmasking System 3: Acidic, 10X, (Cat. No. 927801), the primary antibody was tested at 1:100 dilution overnight at 4°C, followed by incubation with Alexa Fluor® 594 anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
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917901 1 mL 478€
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Description

Melan-A, also known as melanoma antigen recognized by T cells 1 (MART-1), antigen LB39-AA, and antigen SK29-AA, is an 18 kD transmembrane protein that is expressed in a large proportion of melanomas and melanoma cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a MART-1 fusion protein.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution between 1:40 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).

This antibody recognizes MART-1 (Melan-A) [MART1, MART 1] an 18kD transmembrane protein that is expressed in cells of melanocytic lineage. MART-1 is expressed by a large proportion of melanomas and by melanoma cell lines.

In Immunohistochemistry citrate retrieval has also been verified for use with this clone.

Additional Product Notes

Please note, this product (Cat. No. 917901) is sold in concentrated format. For the pre-diluted format of this product, please see Cat. No. 917902.

Application References

(PubMed link indicates BioLegend citation)
  1. Shidham VB, et al. 2001. Am. J. Surg. Pathol. 25:1039.
  2. Fetsch PA, et al. 1999. Cancer Cytopath. 87:37.
  3. Yu LL, et al. 1999. Cancer. 86:617.
  4. Beaty MW, et al. 1997. Cancer (Cancer Cytopathology). 81:57.
  5. Marincola FM, et al. 1996. J. Immunother. Emphasis Tumor Immunol. 19:192.
  6. Rivoltini L, et al. 1995. J. Immunol. 154:2257.
  7. Kawakami Y, et al. 1994. J. Exp. Med. 180:347.
  8. Kawakami Y, et al. 1994. Proc. Natl. Acad, Sci. USA. 91:3515.
RRID
AB_2565202 (BioLegend Cat. No. 917901)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
MHC Antigens
Gene ID
2315 View all products for this Gene ID
UniProt
View information about MART-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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