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Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
W20119E (See other available formats)
Regulatory Status
RUO
Other Names
EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20119E_PURE_CD326_Antibody_1_081922
TE-71 cells were stained with purified anti-mouse CD326 (Ep-CAM) (clone W20119E) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W20119E_PURE_CD326_Antibody_1_081922
    TE-71 cells were stained with purified anti-mouse CD326 (Ep-CAM) (clone W20119E) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W20119E_PURE_CD326_Antibody_2_081922
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD326 (Ep-CAM) (clone W20119E) and Alexa Fluor® 647 anti-mouse Podoplanin (clone PMab-1) (green) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG2b (clone MRG2b-85) (red) for an hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X object.
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112202 100 µg 84€
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Description

Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2924452 (BioLegend Cat. No. 112202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Function
Mediates calcium-independent homophilic cell-cell adhesion
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 08.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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