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Purified anti-mouse CD8b Antibody

Pricing & Availability
Clone
YTS156.7.7 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3, Ly-3
Isotype
Rat IgG2b, κ
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Product Citations
publications
a_YTS156_Pure_031808
C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC
  • a_YTS156_Pure_031808
    C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC
  • b_YTS15677_Purified_CD8b_Antibody_IHCF_060719
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD8b (clone YTS156.7.7) and anti-mouse CD3ε (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 anti-mouse IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • c_YTS156.7.7_Purified_CD8b_Antibody_IF_090612
    C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC
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126602 500 µg 165€
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations
  1. Hummel JF, et al. 2020. Mucosal Immunol. 0.720138889. PubMed
  2. Jangalwe S, et al. 2019. J Immunol. 202:647. PubMed
  3. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  4. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  5. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
RRID
AB_961308 (BioLegend Cat. No. 126602)

Antigen Details

Structure
Ig superfamily, CD8β chain, 34 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8beta on UniProt.org
Go To Top Version: 2    Revision Date: 06.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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