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Purified anti-mouse EOMES Antibody

Pricing & Availability
Clone
W17001A (See other available formats)
Regulatory Status
RUO
Other Names
Eomesodermin, T-box brain protein 2 (TBR-2)
Isotype
Rat IgG2b, λ
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Product Citations
publications
W17001A_PURE_EOMES_Antibody_1_081420
C57BL/6 mouse splenocytes were surface stained with CD8a Alexa Fluor® 488 then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with purified EOMES (clone W17001A) (left) or purified rat IgG2b, λ isotype control (right) followed by anti-rat IgG Alexa Fluor® 647.
  • W17001A_PURE_EOMES_Antibody_1_081420
    C57BL/6 mouse splenocytes were surface stained with CD8a Alexa Fluor® 488 then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with purified EOMES (clone W17001A) (left) or purified rat IgG2b, λ isotype control (right) followed by anti-rat IgG Alexa Fluor® 647.
  • W17001A_PURE_EOMES_Antibody_2_081420
    Whole cell extracts (15 µg total protein) from NK-92 (human positive control), HeLa (human negative control), C57/BL6 primary splenocytes (mouse positive control), and NIH/3T3 (mouse negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-mouse EOMES antibody (clone W17001A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W17001A_PURE_EOMES_Antibody_3_081420
    Whole cell extracts (250 µg total protein) prepared from NK-92 cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) or purified anti-mouse EOMES antibody (clone W17001A). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with W17001A. Lane M: Molecular weight marker.
  • W17001A_PURE_EOMES_Antibody_4_121622
    IHC staining of purified anti-mouse EOMES (clone W17001A) on formalin-fixed paraffin-embedded human colon tumor tissue. Following antigen retrieval using Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor ® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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157702 100 µg 226€
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Description

Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8+ T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8+ T cells. EOMES has been shown to be required to maintain long-term memory of CD8+ T cells and is important for homeostasis of memory and effector T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
WB, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.

RRID
AB_2876538 (BioLegend Cat. No. 157702)

Antigen Details

Distribution

Nucleus

Antigen References
  1. Cooper L, et al. 2018. Plos One. 13(12).
  2. Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54.
  3. Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
Gene ID
13813 View all products for this Gene ID
UniProt
View information about EOMES on UniProt.org
Go To Top Version: 2    Revision Date: 12.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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