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Purified anti-mouse Ly6K Antibody

Pricing & Availability
Clone
MK34 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6K, Ly-6K, mLy-6K
Isotype
Rat IgG2a, κ
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Product Citations
publications
MK34_PURE_Ly6K_Antibody_1_FC_041816
Ly6K transfected-HEK-293 cells were stained with clone MK34 (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • MK34_PURE_Ly6K_Antibody_1_FC_041816
    Ly6K transfected-HEK-293 cells were stained with clone MK34 (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • MK34_PURE_Ly6K_Antibody_2_IHC_041816
    Mouse testicular tissue was formalin-fixed and paraffin-embedded, prior to sectioning. After deparaffinization and antigen retrieval with sodium citrate pH 6, the sample was stained overnight with anti-Ly6k (clone MK34) at 10 µg/ml followed by detection using BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC).
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151302 100 µg 212€
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Description

Lymphocyte antigen 6K (Ly6K) is a 17 kD GPI-anchored membrane protein expressed by testicular germ cells. Ly6K associates with TEX101 and may have a role in spermatogenesis. It is also expressed in some cancer cell types; a recent study also demonstrated its expression in plasma cells. A soluble 12 kD form of Ly6K has been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse testicular homogenate.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1, western blotting1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Maruyama M, et al. 2010. Biochem. Biophys. Res. Commun. 402:75. (IHC-F, IP, WB)
RRID
AB_2616648 (BioLegend Cat. No. 151302)

Antigen Details

Structure
GPI-anchored membrane protein of 17 kD, soluble protein of 12 kD.
Distribution
Testicular germ cells, some cancer cell types, and plasma cells.
Function
Possible role in spermatogenesis.
Interaction
TEX101.
Cell Type
Plasma cells, B cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Antigen References

1. Fujihara Y, et al. 2014. Biol. Reprod. 90:60.
2. von Allmen CE, et al. 2008. Mol. Immunol. 45:2727.
3. Yoshitake H, et al. 2008. Biochem. Biophys. Res. Commun. 372:277.
4. de Nooij-van Dalen AG, et al. 2003. Int. J. Cancer 103:768.

Gene ID
76486 View all products for this Gene ID
UniProt
View information about Ly6K on UniProt.org
Go To Top Version: 1    Revision Date: 04.28.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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