Purified anti-mouse Siglec-G Antibody, Siglec-G, SH1

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Clone: SH1 (See other available formats)
Regulatory Status: RUO
Other Names: Siglec10, Sialic acid-binding Ig-like lectin G
Isotype: Mouse IgG1, κ
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Product Citations: publications

SH1_PURE_Siglec-G_Antibody_032221 — C57 splenocytes were stained with purified Siglec-G (clone SH1) (left) or purified mouse IgG1, ĸ isotype control (right), followed by anti-mouse IgG1 PE and CD19 FITC.

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163302

100 µg

310€

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Description

Siglec-G is a member of the CD33-related Siglec (sialic acid–binding, immunoglobulin-like lectin) family. Siglec-G is the orthologue of human Siglec-10. It is a 110-120 kDa, 688 amino acid type I transmembrane protein and contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs. It is an inhibitory receptor that is expressed in a B cell–restricted way, with particularly high expression in Pre-B and B1a cells. Upon stimulation with an antigen, Siglec-G is recruited to IgM, which is further phosphorylated inhibiting IgM-induced Ca2+ signaling and NFATc1 expression.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2890729 (BioLegend Cat. No. 163302)

Antigen Details

Structure: Type I transmembrane protein that contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs.
Distribution: B cells
Function: Inhibitis IgM-induced Ca2+ signaling and NFATc1 expression
Ligand/Receptor: Lectin
Gene ID: 243958 View all products for this Gene ID
UniProt: View information about Siglec-G on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Siglec-G Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Siglec-G Antibody	SH1	FC
PE anti-mouse Siglec-G	SH1	FC
Alexa Fluor® 647 anti-mouse Siglec-G	SH1	FC
Spark UV™ 387 anti-mouse Siglec-G	SH1	FC
Brilliant Violet 421™ anti-mouse Siglec-G	SH1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.22.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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