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Purified anti-mouse VEGF-A Antibody

Pricing & Availability
Clone
1F07-2C01 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor (VEGF), VEGF-A, VEGFA
Isotype
Rat IgG, κ
Ave. Rating
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Product Citations
publications
1F07dash2C01_PURE_VEGFA_Antibody_WB_050416
Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using anti-mouse VEGF-A (clone 1F07-2C01) antibody.
  • 1F07dash2C01_PURE_VEGFA_Antibody_WB_050416
    Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using anti-mouse VEGF-A (clone 1F07-2C01) antibody.
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512901 50 µg 104€
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512902 200 µg 235€
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Description

VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted glycoproteins that belongs to structurally related superfamily of dimeric growth factors with a cysteine knot motif. This superfamily includes platelet-derived growth factor (PDGF) and transforming growth factor (TGF) family. VEGF-A plays important roles in both vasculogenesis and angiogenesis, including tumor associated angiogenesis and vascular leakage. VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
N-terminal 24 aa sequence of murine VEGF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Immunohistochemistry: Clone 1F07-2C01 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml.

It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655.
  2. Kirsch AH, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed.
Product Citations
  1. Kutikhin AG, et al. 2020. Cells. :9. PubMed
  2. Kirsch A, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed
  3. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  4. Wang L, et al. 2020. Biomed Res Int. 2020:9485398. PubMed
RRID
AB_2212504 (BioLegend Cat. No. 512901)
AB_2212504 (BioLegend Cat. No. 512902)

Antigen Details

Structure
cystine-knot growth factors
Distribution

aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells

Function
angiogenesis
Cell Targets
endothelial cells
Receptors
VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Cell Type
Macrophages
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Contreras A, et al. 2007. Am. J. Transpl. 7:155.
2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109.
3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267.
4. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655
5. Ferrara N,et al. 2003. Nat. Med. 9:669.
6. Shibuya M. 2001. Cell Struct. Funct. 26:25.
7. Senger DR, et al. 1983. Science 219:983.

Regulation
HIF-1, hypoxiaresponsiveness
Gene ID
22339 View all products for this Gene ID
UniProt
View information about VEGF-A on UniProt.org
Go To Top Version: 3    Revision Date: 10.17.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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